PUBLICATION
Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction
- Authors
- Calisesi, G., Candeo, A., Farina, A., D'Andrea, C., Magni, V., Valentini, G., Pistocchi, A., Costa, A., Bassi, A.
- ID
- ZDB-PUB-200731-16
- Date
- 2020
- Source
- Scientific Reports 10: 12771 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 32728161 Full text @ Sci. Rep.
Citation
Calisesi, G., Candeo, A., Farina, A., D'Andrea, C., Magni, V., Valentini, G., Pistocchi, A., Costa, A., Bassi, A. (2020) Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction. Scientific Reports. 10:12771.
Abstract
Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. OPT is affected by spatially variant artifacts caused by the fact that light diffraction is not taken into account by the straight-light propagation models used for reconstruction. These artifacts hinder high-resolution imaging with OPT. In this work we show that, by using a multiview imaging approach, a 3D reconstruction of the bright-field contrast can be obtained without the diffraction artifacts typical of OPT, drastically reducing the amount of acquired data, compared to previously reported approaches. The method, purely based on bright-field contrast of the unstained sample, provides a comprehensive picture of the sample anatomy, as demonstrated in vivo on Arabidopsis thaliana and zebrafish embryos. Furthermore, this bright-field reconstruction can be implemented on practically any multi-view light-sheet fluorescence microscope without complex hardware modifications or calibrations, complementing the fluorescence information with tissue anatomy.
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