PUBLICATION
Tendon Cell Regeneration Is Mediated by Attachment Site-Resident Progenitors and BMP Signaling
- Authors
- Niu, X., Subramanian, A., Hwang, T.H., Schilling, T.F., Galloway, J.L.
- ID
- ZDB-PUB-200711-14
- Date
- 2020
- Source
- Current biology : CB 30(17): 3277-3292.e5 (Journal)
- Registered Authors
- Galloway, Jenna, Schilling, Tom, Subramanian, Arul
- Keywords
- BMP, ablation, nkx2.5, progenitor cells, regeneration, scleraxis, sox10, tendon, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/growth & development*
- Animals, Genetically Modified/metabolism
- Bone Morphogenetic Proteins/genetics
- Bone Morphogenetic Proteins/metabolism*
- Cell Differentiation
- Musculoskeletal System/metabolism*
- Regeneration*
- Stem Cells/cytology*
- Stem Cells/metabolism
- Tendons/cytology*
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism
- PubMed
- 32649909 Full text @ Curr. Biol.
Citation
Niu, X., Subramanian, A., Hwang, T.H., Schilling, T.F., Galloway, J.L. (2020) Tendon Cell Regeneration Is Mediated by Attachment Site-Resident Progenitors and BMP Signaling. Current biology : CB. 30(17):3277-3292.e5.
Abstract
The musculoskeletal system is a striking example of how cell identity and position is coordinated across multiple tissues to ensure function. However, it is unclear upon tissue loss, such as complete loss of cells of a central musculoskeletal connecting tendon, whether neighboring tissues harbor progenitors capable of mediating regeneration. Here, using a zebrafish model, we genetically ablate all embryonic tendon cells and find complete regeneration of tendon structure and pattern. We identify two regenerative progenitor populations, sox10+ perichondrial cells surrounding cartilage and nkx2.5+ cells surrounding muscle. Surprisingly, laser ablation of sox10+ cells, but not nkx2.5+ cells, increases tendon progenitor number in the perichondrium, suggesting a mechanism to regulate attachment location. We find BMP signaling is active in regenerating progenitor cells and is necessary and sufficient for generating new scxa+ cells. Our work shows that muscle and cartilage connective tissues harbor progenitor cells capable of fully regenerating tendons, and this process is regulated by BMP signaling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping