PUBLICATION
Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium
- Authors
- Hansen, J.N., Kaiser, F., Klausen, C., Stüven, B., Chong, R., Bönigk, W., Mick, D.U., Möglich, A., Jurisch-Yaksi, N., Schmidt, F.I., Wachten, D.
- ID
- ZDB-PUB-200625-6
- Date
- 2020
- Source
- eLIFE 9: (Journal)
- Registered Authors
- Jurisch-Yaksi, Nathalie
- Keywords
- cell biology, zebrafish
- MeSH Terms
-
- Animals
- Calcium/metabolism
- Cell Line
- Cilia/physiology*
- Humans
- Mice
- Optogenetics*
- Signal Transduction/physiology*
- Single-Cell Analysis
- Single-Domain Antibodies*
- PubMed
- 32579112 Full text @ Elife
Citation
Hansen, J.N., Kaiser, F., Klausen, C., Stüven, B., Chong, R., Bönigk, W., Mick, D.U., Möglich, A., Jurisch-Yaksi, N., Schmidt, F.I., Wachten, D. (2020) Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium. eLIFE. 9:.
Abstract
Compartmentalization of cellular signaling forms the molecular basis of cellular behavior. The primary cilium constitutes a subcellular compartment that orchestrates signal transduction independent from the cell body. Ciliary dysfunction causes severe diseases, termed ciliopathies. Analyzing ciliary signaling has been challenging due to the lack of tools investigate ciliary signaling. Here, we describe a nanobody-based targeting approach for optogenetic tools in mammalian cells and in vivo in zebrafish to specifically analyze ciliary signaling and function. Thereby, we overcome the loss of protein function observed after fusion to ciliary targeting sequences. We functionally localized modifiers of cAMP signaling, the photo-activated adenylate cyclase bPAC and the light-activated phosphodiesterase LAPD, and the cAMP biosensor mlCNBD-FRET to the cilium. Using this approach, we studied the contribution of spatial cAMP signaling in controlling cilia length. Combining optogenetics with nanobody-based targeting will pave the way to the molecular understanding of ciliary function in health and disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping