PUBLICATION
Zebrafish patient avatars in cancer biology and precision cancer therapy
- Authors
- Fazio, M., Ablain, J., Chuan, Y., Langenau, D.M., Zon, L.I.
- ID
- ZDB-PUB-200422-36
- Date
- 2020
- Source
- Nature reviews. Cancer 20(5): 263-273 (Review)
- Registered Authors
- Langenau, David, Zon, Leonard I.
- Keywords
- none
- MeSH Terms
-
- Animals
- Antineoplastic Agents/pharmacology*
- Disease Models, Animal
- Humans
- Neoplasms/drug therapy*
- Neoplasms/genetics
- Neoplasms/metabolism
- Precision Medicine
- Xenograft Model Antitumor Assays
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 32251397 Full text @ Nat. Rev. Cancer
Citation
Fazio, M., Ablain, J., Chuan, Y., Langenau, D.M., Zon, L.I. (2020) Zebrafish patient avatars in cancer biology and precision cancer therapy. Nature reviews. Cancer. 20(5):263-273.
Abstract
In precision oncology, two major strategies are being pursued for predicting clinically relevant tumour behaviours, such as treatment response and emergence of drug resistance: inference based on genomic, transcriptomic, epigenomic and/or proteomic analysis of patient samples, and phenotypic assays in personalized cancer avatars. The latter approach has historically relied on in vivo mouse xenografts and in vitro organoids or 2D cell cultures. Recent progress in rapid combinatorial genetic modelling, the development of a genetically immunocompromised strain for xenotransplantation of human patient samples in adult zebrafish and the first clinical trial using xenotransplantation in zebrafish larvae for phenotypic testing of drug response bring this tiny vertebrate to the forefront of the precision medicine arena. In this Review, we discuss advances in transgenic and transplantation-based zebrafish cancer avatars, and how these models compare with and complement mouse xenografts and human organoids. We also outline the unique opportunities that these different models present for prediction studies and current challenges they face for future clinical deployment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping