PUBLICATION

Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

Authors
Zampieri, R.M., Adessi, A., Caldara, F., Codato, A., Furlan, M., Rampazzo, C., De Philippis, R., La Rocca, N., Dalla Valle, L.
ID
ZDB-PUB-200422-120
Date
2020
Source
Biomolecules   10(4): (Journal)
Registered Authors
Dalla Valle, Luisa
Keywords
Euganean Thermal District, bioactive molecules, cyanobacteria, exopolysaccharides, inflammation models, zebrafish
MeSH Terms
  • Amputation, Surgical
  • Animal Fins/drug effects
  • Animal Fins/immunology
  • Animals
  • Anti-Inflammatory Agents/pharmacology*
  • Biomarkers/metabolism
  • Cell Survival/drug effects
  • Copper Sulfate/toxicity
  • Dextran Sulfate
  • Disease Models, Animal
  • Fibroblasts/drug effects
  • Fibroblasts/metabolism
  • Gene Expression Regulation/drug effects
  • Humans
  • Inflammation/pathology
  • Monosaccharides/analysis
  • Phormidium/chemistry*
  • Polysaccharides, Bacterial/chemistry
  • Polysaccharides, Bacterial/pharmacology*
  • Temperature*
  • Teratogens/toxicity
  • Zebrafish/embryology
  • Zebrafish/physiology*
PubMed
32290043 Full text @ Biomolecules
Abstract
The Euganean Thermal District (Italy) represents the oldest and largest thermal center in Europe, and its therapeutic mud is considered a unique product whose beneficial effects have been documented since Ancient Roman times. Mud properties depend on the heat and electrolytes of the thermal water, as well as on the bioactive molecules produced by its biotic component, mainly represented by cyanobacteria. The investigation of the healing effects of compounds produced by the Euganean cyanobacteria represents an important goal for scientific validation of Euganean mud therapies and for the discovering of new health beneficial biomolecules. In this work, we evaluated the therapeutic potential of exopolysaccharides (EPS) produced by Phormidium sp. ETS05, the most abundant cyanobacterium of the Euganean mud. Specifically, Phormidium EPS resulted in exerting anti-inflammatory and pro-resolution activities in chemical and injury-induced zebrafish inflammation models as demonstrated using specific transgenic zebrafish lines and morphometric and expression analyses. Moreover, in vivo and in vitro tests showed no toxicity at all for the EPS concentrations tested. The results suggest that these EPS, with their combined anti-inflammatory and pro-resolution activities, could be one of the most important therapeutic molecules present in the Euganean mud and confirm the potential of these treatments for chronic inflammatory disease recovery.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping