PUBLICATION
Contribution of increasing plasma membrane to the energetic cost of early zebrafish embryogenesis
- Authors
- Rodenfels, J., Sartori, P., Golfier, S., Nagendra, K., Neugebauer, K., Howard, J.
- ID
- ZDB-PUB-200213-9
- Date
- 2020
- Source
- Molecular biology of the cell 31(7): 520-526 (Journal)
- Registered Authors
- Neugebauer, Karla
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Count
- Cell Membrane/drug effects
- Cell Membrane/metabolism*
- Embryo, Mammalian/cytology
- Embryo, Mammalian/drug effects
- Embryonic Development*/drug effects
- Energy Metabolism*/drug effects
- Models, Biological
- Nocodazole/pharmacology
- Temperature
- Zebrafish/embryology*
- Zebrafish/metabolism*
- PubMed
- 32049586 Full text @ Mol. Biol. Cell
Citation
Rodenfels, J., Sartori, P., Golfier, S., Nagendra, K., Neugebauer, K., Howard, J. (2020) Contribution of increasing plasma membrane to the energetic cost of early zebrafish embryogenesis. Molecular biology of the cell. 31(7):520-526.
Abstract
How do early embryos allocate the resources stored in the sperm and egg? Recently, we established isothermal calorimetry to measure heat dissipation by living zebrafish embryos and to estimate the energetics of specific developmental events. During the reductive cleavage divisions, the rate of heat dissipation increases from ∼60 nJ s-1 at the 2-cell stage to ∼90 nJ s-1 at the 1024-cell stage. Here we ask, which cellular process(es) drive this increasing energetic cost? We present evidence that the cost is due to the increase in the total surface area of all the cells of the embryo. First, embryo volume stays constant during the cleavage stage, indicating that the increase is not due to growth. Second, the heat increase is blocked by nocodazole, which inhibits DNA replication, mitosis and cell division; this suggests some aspect of cell proliferation contributes to these costs. Third, the heat increases in proportion to the total cell surface area rather than total cell number. Fourth, the heat increase falls within the range of the estimated costs of maintaining and assembling plasma membranes and associated proteins. Thus, the increase in total plasma membrane associated with cell proliferation is likely to contribute appreciably to the total energy budget of the embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping