PUBLICATION
Fast objective coupled planar illumination microscopy
- Authors
- Greer, C.J., Holy, T.E.
- ID
- ZDB-PUB-191004-2
- Date
- 2019
- Source
- Nature communications 10: 4483 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain/diagnostic imaging*
- Diagnostic Imaging/instrumentation*
- Diagnostic Imaging/methods
- Image Processing, Computer-Assisted/instrumentation*
- Image Processing, Computer-Assisted/methods
- Larva
- Luminescent Measurements/instrumentation*
- Luminescent Measurements/methods
- Microscopy/instrumentation*
- Microscopy/methods
- Microscopy, Confocal/instrumentation
- Microscopy, Confocal/methods
- Microscopy, Fluorescence/instrumentation
- Microscopy, Fluorescence/methods
- Microscopy, Fluorescence, Multiphoton/instrumentation
- Microscopy, Fluorescence, Multiphoton/methods
- Reproducibility of Results
- Zebrafish
- PubMed
- 31578369 Full text @ Nat. Commun.
Citation
Greer, C.J., Holy, T.E. (2019) Fast objective coupled planar illumination microscopy. Nature communications. 10:4483.
Abstract
Among optical imaging techniques light sheet fluorescence microscopy is one of the most attractive for capturing high-speed biological dynamics unfolding in three dimensions. The technique is potentially millions of times faster than point-scanning techniques such as two-photon microscopy. However light sheet microscopes are limited by volume scanning rate and/or camera speed. We present speed-optimized Objective Coupled Planar Illumination (OCPI) microscopy, a fast light sheet technique that avoids compromising image quality or photon efficiency. Our fast scan system supports 40 Hz imaging of 700 μm-thick volumes if camera speed is sufficient. We also address the camera speed limitation by introducing Distributed Planar Imaging (DPI), a scaleable technique that parallelizes image acquisition across cameras. Finally, we demonstrate fast calcium imaging of the larval zebrafish brain and find a heartbeat-induced artifact, removable when the imaging rate exceeds 15 Hz. These advances extend the reach of fluorescence microscopy for monitoring fast processes in large volumes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping