ZFIN ID: ZDB-PUB-190906-4
Inhibition of mmp13a during zebrafish fin regeneration disrupts fin growth, osteoblasts differentiation and Laminin organization
Li, L., Zhang, J., Akimenko, M.A.
Date: 2019
Source: Developmental dynamics : an official publication of the American Association of Anatomists   249(2): 187-198 (Journal)
Registered Authors: Akimenko, Marie-Andree
Keywords: actinotrichia, blastema, fin regeneration, mmp13a, morpholino knockdown, osteoblast differentiation
MeSH Terms: none
PubMed: 31487071 Full text @ Dev. Dyn.
ABSTRACT
Matrix metalloproteinases 13 (MMP13) is a potent endopeptidase that regulate cell growth, migration, and extracellular matrix (ECM) remodeling. However, its role in fin regeneration remains unclear.
mmp13a expression is strongly upregulated during blastema formation and persists in the distal blastema. mmp13a knockdown via morpholino electroporation impairs regenerative outgrowth by decreasing cell proliferation, which correlates with a downregulation of fgf10a and sall4 expression in the blastema. Laminin distribution in the basement membrane is also affected in mmp13a MO-injected rays. Another impact of mmp13a knockdown is observed in the skeletal elements of the fin rays. Expression of two main components of actinotrichia, Collagen II and Actinodin1 is highly reduced in mmp13a MO-injected rays leading to highly disorganized actinotrichia pattern. Inhibition of mmp13a strongly affects bone formation as shown by a reduction of Zns5 and sp7 expression and of bone matrix mineralization in rays. These defects are accompanied by a significant increase in apoptosis in mmp13a MO-injected fin regenerates.
Defects of expression of this multifunctional proteinase drastically affects osteoblast differentiation, bone and actinotrichia formation as well as Laminin distribution in the basement membrane of the fin regenerate, suggesting the important role of Mmp13 during the regenerative process. This article is protected by copyright. All rights reserved.
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