PUBLICATION
In Vivo Surface Electrocardiography for Adult Zebrafish
- Authors
- Zhao, Y., Yun, M., Nguyen, S.A., Tran, M., Nguyen, T.P.
- ID
- ZDB-PUB-190820-9
- Date
- 2019
- Source
- Journal of visualized experiments : JoVE (150): (Journal)
- Registered Authors
- Zhao, Yali
- Keywords
- none
- MeSH Terms
-
- Age Factors
- Anesthetics/pharmacology
- Animals
- Cross-Sectional Studies
- Electrocardiography/drug effects
- Electrocardiography/methods*
- Heart Rate/drug effects
- Heart Rate/physiology*
- Humans
- Long QT Syndrome/drug therapy
- Long QT Syndrome/physiopathology
- Male
- Zebrafish/physiology*
- PubMed
- 31424443 Full text @ J. Vis. Exp.
Citation
Zhao, Y., Yun, M., Nguyen, S.A., Tran, M., Nguyen, T.P. (2019) In Vivo Surface Electrocardiography for Adult Zebrafish. Journal of visualized experiments : JoVE. (150).
Abstract
The electrocardiogram waveforms of adult zebrafish and those of humans are remarkably similar. These electrocardiogram similarities enhance the value of zebrafish not only as a research model for human cardiac electrophysiology and myopathies but also as a surrogate model in high throughput pharmaceutical screening for potential cardiotoxicities to humans, such as QT prolongation. As such, in vivo electrocardiography for adult zebrafish is an electrical phenotyping tool that is necessary, if not indispensable, for cross-sectional or longitudinal in vivo electrophysiological characterizations. However, too often, the lack of a reliable, practical, and cost-effective recording method remains a major challenge preventing this in vivo diagnostic tool from becoming more readily accessible. Here, we describe a practical, straightforward approach to in vivo electrocardiography for adult zebrafish using a low-maintenance, cost-effective, and comprehensive system that yields consistent, reliable recordings. We illustrate our protocol using healthy adult male zebrafish of 12-18 months of age. We also introduce a rapid real-time interpretation strategy for quality validation to ensure data accuracy and robustness early in the electrocardiogram recording process.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping