PUBLICATION
Embryonic Exposure to Bisphenol A Impairs Primordial Germ Cell Migration without Jeopardizing Male Breeding Capacity
- Authors
- Lombó, M., Getino-Álvarez, L., Depincé, A., Labbé, C., Herráez, M.P.
- ID
- ZDB-PUB-190729-10
- Date
- 2019
- Source
- Biomolecules 9(8): (Journal)
- Registered Authors
- Keywords
- bisphenol A, epigenetics, primordial germ cells, reproduction, zebrafish
- MeSH Terms
-
- Animals
- Benzhydryl Compounds/pharmacology*
- Breeding
- Cell Movement/drug effects
- Chemokine CXCL12/genetics
- Embryonic Germ Cells/cytology*
- Embryonic Germ Cells/drug effects
- Epigenesis, Genetic/drug effects
- Fertility/drug effects*
- Gene Expression Regulation, Developmental/drug effects
- Male
- Phenols/pharmacology*
- Receptors, CXCR4/genetics
- Testis/drug effects
- Testis/growth & development
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 31349731 Full text @ Biomolecules
- CTD
- 31349731
Citation
Lombó, M., Getino-Álvarez, L., Depincé, A., Labbé, C., Herráez, M.P. (2019) Embryonic Exposure to Bisphenol A Impairs Primordial Germ Cell Migration without Jeopardizing Male Breeding Capacity. Biomolecules. 9(8).
Abstract
A large amount of chemicals are released to the environment each year. Among them, bisphenol A (BPA) is of utmost concern since it interferes with the reproductive system of wild organisms due to its capacity to bind to hormone receptors. Additionally, BPA epigenotoxic activity is known to affect basic processes during embryonic life. However, its effects on primordial germ cells (PGCs) proliferation and migration, both mechanisms being crucial for gametogenesis, remain unknown. To investigate the effects of BPA on PGCs migration and eventual testicle development, zebrafish embryos were exposed to 100, 2000 and 4000 µg/L BPA during the first 24 h of development. Vasa immunostaining of PGCs revealed that exposure to 2000 and 4000 µg/L BPA impaired their migration to the genital ridge. Two pivotal genes of PGCs migration (cxcr4b and sdf1a) were highly dysregulated in embryos exposed to these doses, whereas DNA methylation and epigenetic marks in PGCs and their surrounding somatic cells were not altered. Once embryos reached adulthood, the morphometric study of their gonads revealed that, despite the reduced number of PGCs which colonized the genital ridges, normal testicles were developed. Although H3K9ac decreased in the sperm from treated fishes, it did not affect the progeny development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping