ZFIN ID: ZDB-PUB-190507-45
A Reliable Preclinical Model to Study the Impact of Cigarette Smoke in Development and Disease
Aedo, G., Miranda, M., Chávez, M.N., Allende, M.L., Egaña, J.T.
Date: 2019
Source: Current Protocols in Toxicology   80(1): e78 (Journal)
Registered Authors: Allende, Miguel L.
Keywords: angiogenesis, cigarette smoke extract, tissue regeneration, toxicity, zebrafish
MeSH Terms:
  • Animals
  • Disease Models, Animal
  • Embryo, Nonmammalian/blood supply
  • Embryo, Nonmammalian/drug effects*
  • Larva/drug effects*
  • Neovascularization, Physiologic/drug effects*
  • Tobacco Products*
  • Tobacco Smoke Pollution/adverse effects*
  • Wound Healing/drug effects
  • Zebrafish/growth & development*
PubMed: 31058471 Full text @ Current Protocols in Toxicology
The World Health Organization has estimated that, worldwide, cigarette smoking has caused more than 100 million deaths in the last century, a number that is expected to increase in the future. Understanding cigarette smoke toxicity is key for research and development of proper public health policies. The current challenge is to establish a reliable preclinical model to evaluate the effects of cigarette smoke. In this work, we describe a simple method that allows for quantifying the toxic effects of cigarette smoke using zebrafish. Here, viability of larvae and adult fish, as well as the effects of cigarette smoke extracts on vascular development and tissue regeneration, can be easily assayed. © 2019 by John Wiley & Sons, Inc.