PUBLICATION
Dynamic super-resolution structured illumination imaging in the living brain
- Authors
- Turcotte, R., Liang, Y., Tanimoto, M., Zhang, Q., Li, Z., Koyama, M., Betzig, E., Ji, N.
- ID
- ZDB-PUB-190428-1
- Date
- 2019
- Source
- Proceedings of the National Academy of Sciences of the United States of America 116(19): 9586-9591 (Journal)
- Registered Authors
- Keywords
- adaptive optics, brain imaging, in vivo, super-resolution, synapses
- MeSH Terms
-
- Animals
- Brain/anatomy & histology
- Brain/diagnostic imaging*
- Dendrites/chemistry
- Dendritic Spines/chemistry
- Imaging, Three-Dimensional
- Mice
- Mice, Inbred C57BL
- Microscopy, Fluorescence
- Neuroimaging*
- Zebrafish
- PubMed
- 31028150 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Turcotte, R., Liang, Y., Tanimoto, M., Zhang, Q., Li, Z., Koyama, M., Betzig, E., Ji, N. (2019) Dynamic super-resolution structured illumination imaging in the living brain. Proceedings of the National Academy of Sciences of the United States of America. 116(19):9586-9591.
Abstract
Cells in the brain act as components of extended networks. Therefore, to understand neurobiological processes in a physiological context, it is essential to study them in vivo. Super-resolution microscopy has spatial resolution beyond the diffraction limit, thus promising to provide structural and functional insights that are not accessible with conventional microscopy. However, to apply it to in vivo brain imaging, we must address the challenges of 3D imaging in an optically heterogeneous tissue that is constantly in motion. We optimized image acquisition and reconstruction to combat sample motion and applied adaptive optics to correcting sample-induced optical aberrations in super-resolution structured illumination microscopy (SIM) in vivo. We imaged the brains of live zebrafish larvae and mice and observed the dynamics of dendrites and dendritic spines at nanoscale resolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping