PUBLICATION
A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide
- Authors
- Altan, E., Kubiski, S.V., Boros, Á., Reuter, G., Sadeghi, M., Deng, X., Creighton, E.K., Crim, M.J., Delwart, E.
- ID
- ZDB-PUB-190403-13
- Date
- 2019
- Source
- Zebrafish 16(3): 291-299 (Journal)
- Registered Authors
- Crim, Marcus J.
- Keywords
- , metagenomics, picornavirus, zebrafish
- MeSH Terms
-
- Animals
- Fish Diseases/virology*
- Intestinal Mucosa/virology*
- Picornaviridae/classification*
- Picornaviridae Infections/virology*
- Zebrafish*
- PubMed
- 30939077 Full text @ Zebrafish
Citation
Altan, E., Kubiski, S.V., Boros, Á., Reuter, G., Sadeghi, M., Deng, X., Creighton, E.K., Crim, M.J., Delwart, E. (2019) A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide. Zebrafish. 16(3):291-299.
Abstract
Zebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gut tissue. The closest relatives of this virus showed identity of <20% in their P1 capsids and <36% in their RdRp qualifying zebrafish picornavirus-1 (ZfPV-1) as member of a novel genus with a proposed name of Cyprivirus. Reverse transcription (RT)-PCR testing of zebrafish from North America, Europe, and Asia showed ZfPV-1 to be globally distributed, being detected in 23 of 41 (56%) institutions tested. In situ hybridization of whole zebrafish showed viral RNA was restricted to a subset of enterocytes and cells in the subjacent lamina propria of the intestine and the intestinal mucosa. This naturally occurring and apparently asymptomatic infection (in wild-type zebrafish lineage AB) provides a natural infection system to study picornavirus-host interactions in an advanced vertebrate model organism. Whether ZfPV-1 infection affects any immunological, developmental, or other biological processes in wild-type or mutant zebrafish lineages remains to be determined.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping