PUBLICATION
Generation of motor neurons requires spatiotemporal coordination between retinoic acid and Mib-mediated Notch signaling
- Authors
- Kong, H.J., Ryu, J.H., Kim, J., Kim, J.W., Seong, B., Whang, I., Park, J.Y., Yeo, S.Y.
- ID
- ZDB-PUB-181127-34
- Date
- 2018
- Source
- Animal cells and systems 22: 76-81 (Journal)
- Registered Authors
- Kong, Hee Jeong
- Keywords
- Notch, Zebrafish, mind bomb, motor neuron, retinoic acid
- MeSH Terms
- none
- PubMed
- 30460083 Full text @ Animal Cells Syst (Seoul)
Citation
Kong, H.J., Ryu, J.H., Kim, J., Kim, J.W., Seong, B., Whang, I., Park, J.Y., Yeo, S.Y. (2018) Generation of motor neurons requires spatiotemporal coordination between retinoic acid and Mib-mediated Notch signaling. Animal cells and systems. 22:76-81.
Abstract
Mind bomb (Mib) is an E3 ubiquitin ligase that activates the Notch signaling pathway. A previous study demonstrated that the generation of late-born GABAergic neurons may be regulated by the interplay between Mib and retinoic acid (RA). However, the relationship between Mib function and the retinoid pathway during the generation of late-born motor neurons remains unclear. We investigated the differentiation of neural progenitors into motor neurons by inhibition of Notch signaling and administration of RA to Tg[hsp70-Mib:EGFP] embryos. The number of motor neurons in the ventral spinal cord increased or decreased depending on the temporal inhibition of Mib-mediated Notch signaling. Inhibition of the retinoid pathway by citral treatment had a synergistic effect with overexpression of Mib:EGFP on the generation of ectopic motor neurons. Additionally, the proteolytic fragment of Mib was detected in differentiated P19 cells following treatment with RA. Our observations imply that the function of Mib may be attenuated by the retinoid pathway, and that Mib-mediated Notch signaling and the retinoid pathway play critical roles in the spatiotemporal differentiation of motor neurons.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping