PUBLICATION
Single-cell analyses identify distinct and intermediate states of zebrafish pancreatic islet development
- Authors
- Lu, C.J., Fan, X.Y., Guo, Y.F., Cheng, Z.C., Dong, J., Chen, J.Z., Li, L.Y., Wang, M.W., Wu, Z.K., Wang, F., Tong, X.J., Luo, L.F., Tang, F.C., Zhu, Z.Y., Zhang, B.
- ID
- ZDB-PUB-181109-6
- Date
- 2018
- Source
- Journal of molecular cell biology 11(6): 435-447 (Journal)
- Registered Authors
- Zhang, Bo
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/embryology
- Animals, Genetically Modified/genetics
- Antigens, Differentiation/biosynthesis*
- Antigens, Differentiation/genetics
- Gene Expression Regulation, Developmental*
- Islets of Langerhans/embryology*
- Single-Cell Analysis*
- Transcription, Genetic*
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 30407522 Full text @ J. Mol. Cell Biol.
Citation
Lu, C.J., Fan, X.Y., Guo, Y.F., Cheng, Z.C., Dong, J., Chen, J.Z., Li, L.Y., Wang, M.W., Wu, Z.K., Wang, F., Tong, X.J., Luo, L.F., Tang, F.C., Zhu, Z.Y., Zhang, B. (2018) Single-cell analyses identify distinct and intermediate states of zebrafish pancreatic islet development. Journal of molecular cell biology. 11(6):435-447.
Abstract
Pancreatic endocrine islets are vital for glucose homeostasis. However, the islet developmental trajectory and its regulatory network are not well understood. To define the features of these specification and differentiation processes, we isolated individual islet cells from TgBAC(neurod1:EGFP) transgenic zebrafish and analyzed islet developmental dynamics across four different embryonic stages using a single-cell RNA-seq strategy. We identified proliferative endocrine progenitors, which could be further categorized by different cell cycle phases with the G1/S sub-population displaying a distinct differentiation potential. We identified endocrine precursors, a heterogeneous intermediate-state population consisting of lineage-primed alpha, beta and delta cells that were characterized by the expression of lineage-specific transcription factors and relatively low expression of terminally differentiation markers. The terminally differentiated alpha, beta and delta cells displayed stage-dependent differentiation states, which were related to their functional maturation. Our data unveiled distinct states, events and molecular features during the islet developmental transition, and provided resources to comprehensively understand the lineage hierarchy of islet development at the single-cell level.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping