PUBLICATION
Zebrafish knock-ins swim into the mainstream
- Authors
- Prykhozhij, S.V., Berman, J.N.
- ID
- ZDB-PUB-181028-2
- Date
- 2018
- Source
- Disease models & mechanisms 11(10): (Review)
- Registered Authors
- Berman, Jason, Prykhozhij, Sergey
- Keywords
- CRISPR/Cas9, Genome editing, Point mutations, Zebrafish
- MeSH Terms
-
- Animals
- Point Mutation/genetics
- Zebrafish/genetics*
- CRISPR-Cas Systems/genetics
- Disease Models, Animal
- Gene Knock-In Techniques*
- PubMed
- 30366936 Full text @ Dis. Model. Mech.
Citation
Prykhozhij, S.V., Berman, J.N. (2018) Zebrafish knock-ins swim into the mainstream. Disease models & mechanisms. 11(10).
Abstract
The zebrafish is an increasingly popular model organism for human genetic disease research. CRISPR/Cas9-based approaches are currently used for multiple gene-editing purposes in zebrafish, but few studies have developed reliable ways to introduce precise mutations. Point mutation knock-in using CRISPR/Cas9 and single-stranded oligodeoxynucleotides (ssODNs) is currently the most promising technology for this purpose. Despite some progress in applying this technique to zebrafish, there is still a great need for improvements in terms of its efficiency, optimal design of sgRNA and ssODNs and broader applicability. The papers discussed in this Editorial provide excellent case studies on identifying problems inherent in the mutation knock-in technique, quantifying these issues and proposing strategies to overcome them. These reports also illustrate how the procedures for introducing specific mutations can be straightforward, such that ssODNs with only the target mutation are sufficient for generating the intended knock-in animals. Two of the studies also develop interesting point mutant knock-in models for cardiac diseases, validating the translational relevance of generating knock-in mutations and opening the door to many possibilities for their further study.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping