PUBLICATION
Pupil mask diversity for image correction in microscopy
- Authors
- Wilding, D., Pozzi, P., Soloviev, O., Vdovin, G., Verhaegen, M.
- ID
- ZDB-PUB-180820-2
- Date
- 2018
- Source
- Optics express 26: 14832-14841 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 30114789 Full text @ Opt. Express
Citation
Wilding, D., Pozzi, P., Soloviev, O., Vdovin, G., Verhaegen, M. (2018) Pupil mask diversity for image correction in microscopy. Optics express. 26:14832-14841.
Abstract
Three-dimensional microscopy suffers from sample-induced aberrations that reduce the resolution and lead to misinterpretations of the object distribution. In this paper, the resolution of a three-dimensional fluorescent microscope is significantly improved by introducing an amplitude diversity in the form of a binary amplitude mask positioned in several different orientations within the pupil, followed by computer processing of the diversity images. The method has proved to be fast, easy to implement, and cost-effective in high-resolution imaging of casper fli:GFP zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping