PUBLICATION
Aroclor 1254 and BDE-47 inhibit dopaminergic function manifesting as changes in locomotion behaviors in zebrafish embryos
- Authors
- Tanaka, Y., Fujiwara, M., Shindo, A., Yin, G., Kitazawa, T., Teraoka, H.
- ID
- ZDB-PUB-180609-1
- Date
- 2018
- Source
- Chemosphere 193: 1207-1215 (Journal)
- Registered Authors
- Teraoka, Hiroki
- Keywords
- Catecholamine, Motor movement, Non-coplanar PCB, PBDE, Zebrafish
- MeSH Terms
-
- Animals
- Chlorodiphenyl (54% Chlorine)/pharmacology
- Chlorodiphenyl (54% Chlorine)/therapeutic use*
- Halogenated Diphenyl Ethers/pharmacology
- Halogenated Diphenyl Ethers/therapeutic use*
- Polychlorinated Biphenyls/adverse effects*
- Zebrafish/embryology*
- PubMed
- 29874750 Full text @ Chemosphere
Citation
Tanaka, Y., Fujiwara, M., Shindo, A., Yin, G., Kitazawa, T., Teraoka, H. (2018) Aroclor 1254 and BDE-47 inhibit dopaminergic function manifesting as changes in locomotion behaviors in zebrafish embryos. Chemosphere. 193:1207-1215.
Abstract
Contamination with polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) in the environment is a major concern due to their persistent bioaccumulative toxicity that can disturb neurobehavioral functions including movements. Recently, it was reported that some PBDE including BDE-47 stimulates locomotor activities of zebrafish embryos by unknown mechanism. In this study, motor movements of the zebrafish embryo were used as a model system to evaluate the neuronal toxicity of a non-coplanar PCB-dominant mixture (Aroclor 1254) and BDE-47. Both organohalogens increased tail shaking and rotation of embryos in a concentration-dependent manner. Chemical inhibition and gene knock-down of tyrosine hydroxylase and vesicular monoamine transporter 2 (VMAT2) also induced hyperactivities. Hyperactivities induced by these treatments were all inhibited by supplementation of l-tyrosine and l-dopa, precursors of dopamine synthesis. Both organohalogens reduced dopamine contents and increased the 3,4-dihydroxyphenylacetic acid (DOPAC)/dopamine ratio in whole embryos. The results suggest that functional inhibition of dopaminergic neurons is involved in hyperactivities of zebrafish embryos caused by Aroclor 1254 and BDE-47.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping