PUBLICATION

Investigation of the molecular mechanisms of hepatic injury upon naphthalene exposure in zebrafish (Danio rerio)

Authors
Chen, H., Sheng, L., Gong, Z., Ru, S., Bian, H.
ID
ZDB-PUB-180512-3
Date
2018
Source
Ecotoxicology (London, England)   27(6): 650-660 (Journal)
Registered Authors
Gong, Zhiyuan
Keywords
Histology, Microarray, Naphthalene, Transgenic-Zebrafish, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/metabolism
  • Female
  • Fish Proteins/genetics
  • Fish Proteins/metabolism
  • Gene Expression/drug effects*
  • Insecticides/toxicity*
  • Liver/drug effects*
  • Naphthalenes/toxicity*
  • Neutrophils/drug effects
  • Neutrophils/metabolism*
  • Oligonucleotide Array Sequence Analysis
  • Organ Size/drug effects
  • Toxicity Tests, Subacute
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed
29748829 Full text @ Ecotoxicology
Abstract
Naphthalene has been used worldwide as a commercial insecticide for decades, which when detected in the environment can have various negative effects on non-target organism, such as hepatotoxicity. However, the molecular mechanisms of how naphthalene acts to affect the liver in zebrafish (Danio rerio) remains unknown. In this study, we evaluated the potential toxic effects of naphthalene on livers in female adult zebrafish over a 21-day subacute exposure. Global hepatic gene expression was examined by microarrays and the results indicated the regulated genes were associated significantly with vital hepatic injury pathways and GO categories upon naphthalene exposure, such as disruptions in lipid metabolism, inflammatory response, and the carcinogenic processes. According to our observations of liver histology, nuclear enlargement as a potential indicator of cancers and hepatic lipometabolic disorder precisely were supported. The 96 h acute naphthalene tests on Tg(lysC:DsRed) and LiPan lines larvae revealed recruitment of neutrophils by the liver, as well as decreased liver size, which further confirmed hepatic inflammation response to naphthalene exposure. Thus, these findings advance the field of ecotoxicology by unveiling a new role of naphthalene as a leading cause of liver damage and provide potential biomarker-genes for environmental naphthalene monitoring.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping