PUBLICATION

A noncanonical function of histidyl-tRNA synthetase: inhibition of vascular hyperbranching during zebrafish development

Authors
Ni, R., Luo, L.
ID
ZDB-PUB-180511-5
Date
2018
Source
FEBS Open Bio   8: 722-731 (Journal)
Registered Authors
Luo, Lingfei
Keywords
Cdh5, Vegfa, angiogenesis, histidyl‐tRNA synthetase, zebrafish
MeSH Terms
none
PubMed
29744287 Full text @ FEBS Open Bio
Abstract
Histidyl-tRNA synthetase (Hars) catalyzes the ligation of histidine residues to cognate tRNA. Here, we demonstrate a noncanonical function of Hars in vascular development in zebrafish. We obtained a novel zebrafish cq34 mutant which exhibited hyperbranching of cranial and intersegmental blood vessels 48 h after fertilization. The gene responsible for this phenotype was identified as hars. We found the increased expression of cdh5 and vegfa in the harscq34 mutant. Knockdown of cdh5 in the mutant reduced disordered connections of the hindbrain capillaries. Inhibition of vascular endothelial growth factor signaling suppressed the abnormal vascular branching observed in the mutant. Moreover, the human HARS mRNA rescued the vascular defects in the cq34 mutant. Thus, the noncanonical function of Hars regulates vascular development, mainly by modulating expression of cdh5 and vegfa.
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Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
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Orthology
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Errata and Notes