PUBLICATION
50 Hz volumetric functional imaging with continuously adjustable depth of focus
- Authors
- Lu, R., Tanimoto, M., Koyama, M., Ji, N.
- ID
- ZDB-PUB-180421-15
- Date
- 2018
- Source
- Biomedical Optics Express 9: 1964-1976 (Journal)
- Registered Authors
- Keywords
- (110.0110) Imaging systems, (170.2520) Fluorescence microscopy, (170.2655) Functional monitoring and imaging, (180.0180) Microscopy
- MeSH Terms
- none
- PubMed
- 29675332 Full text @ Biomed. Opt. Express
Citation
Lu, R., Tanimoto, M., Koyama, M., Ji, N. (2018) 50 Hz volumetric functional imaging with continuously adjustable depth of focus. Biomedical Optics Express. 9:1964-1976.
Abstract
Understanding how neural circuits control behavior requires monitoring a large population of neurons with high spatial resolution and volume rate. Here we report an axicon-based Bessel beam module with continuously adjustable depth of focus (CADoF), that turns frame rate into volume rate by extending the excitation focus in the axial direction while maintaining high lateral resolutions. Cost-effective and compact, this CADoF Bessel module can be easily integrated into existing two-photon fluorescence microscopes. Simply translating one of the relay lenses along its optical axis enabled continuous adjustment of the axial length of the Bessel focus. We used this module to simultaneously monitor activity of spinal projection neurons extending over 60 µm depth in larval zebrafish at 50 Hz volume rate with adjustable axial extent of the imaged volume.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping