PUBLICATION
Hypomethylation of GRHL3 gene is associated with the occurrence of neural tube defects
- Authors
- Tian, T., Wang, L., Shen, Y., Zhang, B., Finnell, R.H., Ren, A.
- ID
- ZDB-PUB-180329-2
- Date
- 2018
- Source
- Epigenomics 10(7): 891-901 (Journal)
- Registered Authors
- Shen, Yan, Tian, Tian, Zhang, Bo
- Keywords
- GRHL3 gene, PCP pathway, methylation, neural tube defects
- MeSH Terms
-
- Animals
- Case-Control Studies
- CpG Islands
- DNA Methylation
- DNA-Binding Proteins/genetics*
- Fetus
- Humans
- Neural Tube Defects/genetics*
- Transcription Factors/genetics*
- Zebrafish Proteins/genetics
- PubMed
- 29587534 Full text @ Epigenomics
Citation
Tian, T., Wang, L., Shen, Y., Zhang, B., Finnell, R.H., Ren, A. (2018) Hypomethylation of GRHL3 gene is associated with the occurrence of neural tube defects. Epigenomics. 10(7):891-901.
Abstract
Aim To investigate the relationship between GRHL3 methylation and the etiology of neural tube defects (NTDs).
Materials & methods Analyze data from a genome-wide DNA methylation array. Targeted DNA methylation analysis was performed for 46 cases and 23 controls. At last, grhl3 overexpression and gene depletion experiments were conducted in zebrafish.
Results Five hypomethylated CpGs were discovered in the methylation arrays performed on NTD cases. In a validation study, 15 hypomethylated CpGs were found and the overall methylation levels decreased in brain/spinal cord tissue from NTD cases. The knockdown and overexpression of grhl3 in zebrafish damaged embryonic convergent extension processes.
Conclusion Hypomethylation of GRHL3 in central nervous tissue is associated with NTDs, further supporting the importance of GRHL3 and methylation in proper neural tube closure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping