ZFIN ID: ZDB-PUB-180321-32
Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)
Sander, J.D., Dahlborg, E.J., Goodwin, M.J., Cade, L., Zhang, F., Cifuentes, D., Curtin, S.J., Blackburn, J.S., Thibodeau-Beganny, S., Qi, Y., Pierick, C.J., Hoffman, E., Maeder, M.L., Khayter, C., Reyon, D., Dobbs, D., Langenau, D.M., Stupar, R.M., Giraldez, A.J., Voytas, D.F., Peterson, R.T., Yeh, J.R., Joung, J.K.
Date: 2011
Source: Nature Methods 8: 67-9 (Journal)
Registered Authors: Cifuentes, Daniel, Giraldez, Antonio, Hoffman, Ellen, Langenau, David, Peterson, Randall, Yeh, Jing-Ruey (Joanna)
Keywords: none
MeSH Terms:
  • Animals
  • Arabidopsis/genetics
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Endonucleases/genetics*
  • Endonucleases/metabolism*
  • Genome
  • Protein Engineering*
  • Soybeans/genetics
  • Zebrafish/genetics
  • Zinc Fingers/genetics
  • Zinc Fingers/physiology*
PubMed: 21151135 Full text @ Nat. Methods
ABSTRACT
Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe context-dependent assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA-generated ZFNs, we rapidly altered 20 genes in Danio rerio, Arabidopsis thaliana and Glycine max. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multigene pathways or genome-wide alterations.
ADDITIONAL INFORMATION