ZFIN ID: ZDB-PUB-171111-6
Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish
Lagendijk, A.K., Gomez, G.A., Baek, S., Hesselson, D., Hughes, W.E., Paterson, S., Conway, D.E., Belting, H.G., Affolter, M., Smith, K.A., Schwartz, M.A., Yap, A.S., Hogan, B.M.
Date: 2017
Source: Nature communications   8: 1402 (Journal)
Registered Authors: Affolter, Markus, Belting, Heinz-Georg Paul (Henry), Hesselson, Daniel, Hogan, Ben M., Paterson, Scott, Smith, Kelly
Keywords: none
MeSH Terms:
  • Actomyosin/physiology
  • Animals
  • Antigens, CD/genetics
  • Antigens, CD/physiology*
  • Aorta/embryology
  • Biomechanical Phenomena
  • Cadherins/genetics
  • Cadherins/physiology*
  • Fluorescence Resonance Energy Transfer
  • Intercellular Junctions/physiology
  • Mechanotransduction, Cellular/physiology
  • Molecular Imaging
  • Mutation
  • Neovascularization, Physiologic/genetics
  • Tensile Strength/physiology
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed: 29123087 Full text @ Nat. Commun.
Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development.