ZFIN ID: ZDB-PUB-171107-14
Osteoblast Production by Reserved Progenitor Cells in Zebrafish Bone Regeneration and Maintenance
Ando, K., Shibata, E., Hans, S., Brand, M., Kawakami, A.
Date: 2017
Source: Developmental Cell   43(5): 643-650.e3 (Journal)
Registered Authors: Ando, Kazunori, Brand, Michael, Hans, Stefan, Kawakami, Atsushi
Keywords: bone regeneration, cell ablation, cre-loxP, fin, matrix metalloproteinase 9, osteoblast progenitor cells, stem cells, transplantation, zebrafish
MeSH Terms:
  • Animals
  • Bone Regeneration/physiology*
  • Bone and Bones/cytology*
  • Cell Dedifferentiation/physiology
  • Cell Lineage/physiology
  • Cell Proliferation/physiology*
  • Osteoblasts/cytology*
  • Regeneration/physiology*
  • Stem Cells/cytology*
  • Zebrafish*/metabolism
  • Zebrafish Proteins/metabolism
PubMed: 29103952 Full text @ Dev. Cell
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ABSTRACT
Mammals cannot re-form heavily damaged bones as in large fracture gaps, whereas zebrafish efficiently regenerate bones even after amputation of appendages. However, the source of osteoblasts that mediate appendage regeneration is controversial. Several studies in zebrafish have shown that osteoblasts are generated by dedifferentiation of existing osteoblasts at injured sites, but other observations suggest that de novo production of osteoblasts also occurs. In this study, we found from cell-lineage tracing and ablation experiments that a group of cells reserved in niches serves as osteoblast progenitor cells (OPCs) and has a significant role in fin ray regeneration. Besides regeneration, OPCs also supply osteoblasts for normal bone maintenance. We further showed that OPCs are derived from embryonic somites, as is the case with embryonic osteoblasts, and are replenished from mesenchymal precursors in adult zebrafish. Our findings reveal that reserved progenitors are a significant and complementary source of osteoblasts for zebrafish bone regeneration.
ADDITIONAL INFORMATION