ZFIN ID: ZDB-PUB-171031-5
High-Throughput Automated Chemical Screens in Zebrafish
Saydmohammed, M., Tsang, M.
Date: 2018
Source: Methods in molecular biology (Clifton, N.J.)   1683: 383-393 (Chapter)
Registered Authors: Tsang, Michael
Keywords: Chemical genetics, Drug discovery, FGF hyper-activators, Small molecule screens, Transgenic reporter
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Biomarkers
  • Drug Discovery*/methods
  • Embryo, Nonmammalian/drug effects
  • Gene Expression
  • Genes, Reporter
  • High-Throughput Screening Assays*
  • Microscopy, Fluorescence
  • Small Molecule Libraries
  • Zebrafish
PubMed: 29082504 Full text @ Meth. Mol. Biol.
Zebrafish are increasingly used to perform phenotypic screens to identify agents that can alter physiology in a whole organismal context. Here, we describe an automated high-content chemical screen using transgenic zebrafish embryos to identify small molecules that modulate Fibroblast Growth Factor Signaling. High content multi-well screening was further refined with a particular emphasis on automated imaging and quantification that increases sensitivity and throughput of whole organism chemical screens.