|ZFIN ID: ZDB-PUB-170922-15|
Muscle stem cells and regeneration in zebrafish models of muscular dystrophies
Hall, T.E., Bryson-Richardson, R.J., Cole, N.J., Currie, P.D.
|Source:||Mechanisms of Development 122: S131 (Abstract)|
|Registered Authors:||Bryson-Richardson, Robert, Cole, Nicholas, Currie, Peter D., Hall, Thomas|
Hall, T.E., Bryson-Richardson, R.J., Cole, N.J., Currie, P.D. (2005) Muscle stem cells and regeneration in zebrafish models of muscular dystrophies. Mechanisms of Development. 122:S131.
ABSTRACTThe growth, repair and regeneration of skeletal muscle is dependent upon a population of mononuclear precursor cells known as satellite cells. Recently, the classical view of skeletal muscle satellite cells as a homogeneous, monopotent population has been challenged by the discovery of multipotent, muscle derived adult stem cells coexisting within the myotome. Furthermore, several studies now suggest that satellite cells comprise separate sub-populations exhibiting distinct biochemical and biological properties. As such, many important questions are now posed as to the normal role and function of these cellular compartments, as well as their potential utility in cell-mediated therapies. We are currently applying the power of the zebrafish genetic model to the study of skeletal muscle stem cells for the first time, to answer questions which have hitherto been difficult or impossible to address in other systems. We are performing a comprehensive “cradle to grave” in situ hybridisation and immunohistochemistry screen using zebrafish homologues of known mammalian satellite cell (e.g. Pax7, syndecan 3/4, cmet) and stem cell markers (e.g. Sca-1, c-kit, CD45). Expression patterns from wild-type fish are also being compared with those from the sapje model of Duchenne’s muscular dystrophy using a multi-dimensional scaling approach.
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