PUBLICATION
The first wave of T lymphopoiesis in zebrafish arises from aorta endothelium independent of hematopoietic stem cells
- Authors
- Tian, Y., Xu, J., Feng, S., He, S., Zhao, S., Zhu, L., Jin, W., Dai, Y., Luo, L., Qu, J.Y., Wen, Z.
- ID
- ZDB-PUB-170922-11
- Date
- 2017
- Source
- The Journal of experimental medicine 214(11): 3347-3360 (Journal)
- Registered Authors
- Luo, Lingfei, Wen, Zilong
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Aorta/cytology*
- Aorta/embryology
- Aorta/metabolism
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Endothelium, Vascular/cytology*
- Endothelium, Vascular/embryology
- Endothelium, Vascular/metabolism
- Gene Expression Regulation, Developmental
- Gonads/cytology
- Gonads/embryology
- Gonads/metabolism
- Hematopoietic Stem Cells/cytology*
- Hematopoietic Stem Cells/metabolism
- In Situ Hybridization
- Lymphopoiesis*
- Mesonephros/cytology
- Mesonephros/embryology
- Mesonephros/metabolism
- Microscopy, Confocal
- T-Lymphocytes/cytology*
- T-Lymphocytes/metabolism
- Time-Lapse Imaging/methods
- Zebrafish
- PubMed
- 28931624 Full text @ J. Exp. Med.
Citation
Tian, Y., Xu, J., Feng, S., He, S., Zhao, S., Zhu, L., Jin, W., Dai, Y., Luo, L., Qu, J.Y., Wen, Z. (2017) The first wave of T lymphopoiesis in zebrafish arises from aorta endothelium independent of hematopoietic stem cells. The Journal of experimental medicine. 214(11):3347-3360.
Abstract
T lymphocytes are key cellular components of the adaptive immune system and play a central role in cell-mediated immunity in vertebrates. Despite their heterogeneities, it is believed that all different types of T lymphocytes are generated exclusively via the differentiation of hematopoietic stem cells (HSCs). Using temporal-spatial resolved fate-mapping analysis and time-lapse imaging, here we show that the ventral endothelium in the zebrafish aorta-gonad-mesonephros and posterior blood island, the hematopoietic tissues previously known to generate HSCs and erythromyeloid progenitors, respectively, gives rise to a transient wave of T lymphopoiesis independent of HSCs. This HSC-independent T lymphopoiesis occurs early and generates predominantly CD4 Tαβ cells in the larval but not juvenile and adult stages, whereas HSC-dependent T lymphopoiesis emerges late and produces various subtypes of T lymphocytes continuously from the larval stage to adulthood. Our study unveils the existence, origin, and ontogeny of HSC-independent T lymphopoiesis in vivo and reveals the complexity of the endothelial-hematopoietic transition of the aorta.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping