Muscle Stem Cells Undergo Extensive Clonal Drift during Tissue Growth via Meox1-Mediated Induction of G2 Cell-Cycle Arrest
- Nguyen, P.D., Gurevich, D.B., Sonntag, C., Hersey, L., Alaei, S., Nim, H.T., Siegel, A., Hall, T.E., Rossello, F.J., Boyd, S.E., Polo, J.M., Currie, P.D.
- Cell Stem Cell 21: 107-119.e6 (Journal)
- Registered Authors
- Alaei, Sara, Currie, Peter D., Gurevich, David, Hall, Thomas, Hersey, Lucy, Nguyen, Phong D., Siegel, Ashley, Sonntag, Carmen
- cell cycle, clonal drift, growth, imaging, skeletal muscle, stem cell, zebrafish
- MeSH Terms
- Cell Line
- Cell Lineage/physiology*
- Cyclin B1/genetics
- Cyclin B1/metabolism
- G2 Phase Cell Cycle Checkpoints/physiology*
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- 28686860 Full text @ Cell Stem Cell
Nguyen, P.D., Gurevich, D.B., Sonntag, C., Hersey, L., Alaei, S., Nim, H.T., Siegel, A., Hall, T.E., Rossello, F.J., Boyd, S.E., Polo, J.M., Currie, P.D. (2017) Muscle Stem Cells Undergo Extensive Clonal Drift during Tissue Growth via Meox1-Mediated Induction of G2 Cell-Cycle Arrest. Cell Stem Cell. 21:107-119.e6.
Organ growth requires a careful balance between stem cell self-renewal and lineage commitment to ensure proper tissue expansion. The cellular and molecular mechanisms that mediate this balance are unresolved in most organs, including skeletal muscle. Here we identify a long-lived stem cell pool that mediates growth of the zebrafish myotome. This population exhibits extensive clonal drift, shifting from random deployment of stem cells during development to reliance on a small number of dominant clones to fuel the vast majority of muscle growth. This clonal drift requires Meox1, a homeobox protein that directly inhibits the cell-cycle checkpoint gene ccnb1. Meox1 initiates G2 cell-cycle arrest within muscle stem cells, and disrupting this G2 arrest causes premature lineage commitment and the resulting defects in muscle growth. These findings reveal that distinct regulatory mechanisms orchestrate stem cell dynamics during organ growth, beyond the G0/G1 cell-cycle inhibition traditionally associated with maintaining tissue-resident stem cells.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes