Zhang, Y., Zhang, C., Peng, G., Duan, X. (2014) Characteristics of craniofacial bone development of zebrafish. Journal of Oral Science Research. 30(2):97-99.
Objective:To study the development characteristics of zebrafish calvarial bones in the different developmental stages and compare two double-staining methods.Methods:The zebrafish were collected from 3dto 45d and stained with A and B methods for the bone staining.The staining results were observed with stereomicroscope. Results:Both methods showed no difference in cartilage staining,but bone staining results with B methods was better in zebrafish older than 15d.The craniofacial bones gradually became calcified in a temporal and spatial order. Conclusion:The temporal and spatial calcification characteristics of craniofacial bone development of zebrafish enable us to study the human craniofacial diseases.Further studies with zebrafish model should be carried out in the basic oral medicine research.