ZFIN ID: ZDB-PUB-170612-4
Characteristics of craniofacial bone development of zebrafish
Zhang, Y., Zhang, C., Peng, G., Duan, X.
Date: 2014
Source: Journal of Oral Science Research 30(2): 97-99 (Journal)
Registered Authors: Duan, Xiaohong, Peng, Gang, Zhang, Cuizhen, Zhang, Yanli
Keywords: Alcian blue, Alizarin red, Two-color staining, Zebrafish
MeSH Terms: none
PubMed: none
Objective:To study the development characteristics of zebrafish calvarial bones in the different developmental stages and compare two double-staining methods.Methods:The zebrafish were collected from 3dto 45d and stained with A and B methods for the bone staining.The staining results were observed with stereomicroscope. Results:Both methods showed no difference in cartilage staining,but bone staining results with B methods was better in zebrafish older than 15d.The craniofacial bones gradually became calcified in a temporal and spatial order. Conclusion:The temporal and spatial calcification characteristics of craniofacial bone development of zebrafish enable us to study the human craniofacial diseases.Further studies with zebrafish model should be carried out in the basic oral medicine research.
[Article in Chinese.]