The dual developmental origin of spinal cerebrospinal fluid-contacting neurons gives rise to distinct functional subtypes

Djenoune, L., Desban, L., Gomez, J., Sternberg, J.R., Prendergast, A., Langui, D., Quan, F.B., Marnas, H., Auer, T.O., Rio, J.P., Del Bene, F., Bardet, P.L., Wyart, C.
Scientific Reports   7: 719 (Journal)
Registered Authors
Auer, Thomas, Bardet, Pierre-Luc, Del Bene, Filippo, Djenoune, Lydia, Prendergast, Andrew, Wyart, Claire
Cellular neuroscience, Neurophysiology
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Axons/physiology
  • Axons/ultrastructure
  • Biomarkers
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism
  • Cell Differentiation
  • Cerebrospinal Fluid/metabolism*
  • Fluorescent Antibody Technique
  • Ganglia, Spinal
  • Homozygote
  • Mutation
  • Neurons/physiology*
  • Neurons/ultrastructure
  • Sensory Receptor Cells/physiology
  • Sensory Receptor Cells/ultrastructure
  • Signal Transduction*
  • Spinal Cord/cytology*
  • Spinal Cord/physiology*
  • Spinal Nerve Roots
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
28389647 Full text @ Sci. Rep.
Chemical and mechanical cues from the cerebrospinal fluid (CSF) can affect the development and function of the central nervous system (CNS). How such cues are detected and relayed to the CNS remains elusive. Cerebrospinal fluid-contacting neurons (CSF-cNs) situated at the interface between the CSF and the CNS are ideally located to convey such information to local networks. In the spinal cord, these GABAergic neurons expressing the PKD2L1 channel extend an apical extension into the CSF and an ascending axon in the spinal cord. In zebrafish and mouse spinal CSF-cNs originate from two distinct progenitor domains characterized by distinct cascades of transcription factors. Here we ask whether these neurons with different developmental origins differentiate into cells types with different functional properties. We show in zebrafish larva that the expression of specific markers, the morphology of the apical extension and axonal projections, as well as the neuronal targets contacted by CSF-cN axons, distinguish the two CSF-cN subtypes. Altogether our study demonstrates that the developmental origins of spinal CSF-cNs give rise to two distinct functional populations of sensory neurons. This work opens novel avenues to understand how these subtypes may carry distinct functions related to development of the spinal cord, locomotion and posture.
Genes / Markers
Show all Figures
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes