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Research
General Information
ZIRC
ZFIN ID: ZDB-PERS-110606-1
Wyart, Claire
Email: claire.wyart@icm-institute.org
URL: http://www.wyartlab.org
Affiliation: Wyart Lab
Address: Brain and Spine Institute (Institut du Cerveau et de la Moelle epinere, ICM) INSERM U975, CNRS UMR 7225 Universite Pierre et Marie Curie UMP-S975 Hôpital Salpetriere - Batiment ICM - 47, bld de l'hôpital Paris, 75013 France
Country: France
Phone: +33-1-5727-4310
Fax: +33-1-5727-4027
Orcid ID:


BIOGRAPHY AND RESEARCH INTERESTS
Neurobiologists have been dissecting vertebrates for centuries, describing the anatomy of different types of cells in the spinal cord. They have proposed that these cells constitute a central pattern generator underlying the rhythmic bursting activity needed for locomotion. How specific cell types are recruited to generate specific locomotor pattern (with different speed, direction and gating), is still unknown. By developing light gated channels to remotely control (activate or inhibit) neurons in genetically identified neurons in an awake behaving animals (also called “optogenetics”), we have a way to test for the first time the direct relevance of specific neuronal type in underlying specific locomotor behaviors.Our team has developed in vivo and has applied this approach to tackle a long-standing question: the function of cerebrospinal fluid contacting neurons (CSFns) in the spinal cord of Vertebrates. We have been able to demonstrate that KA cells are able to trigger specifically slow locomotion at early stage of development. The fact that there is an apparent homologue of the KA neuron in mammals suggests that there could be a general modulatory principle for vertebrate locomotion. Now our team aims at reconstructing the circuit of slow swimming involved in the CSFns mediated response, and more generally dissect the underlying circuits dynamically forming the multiple central pattern generators which are dynamically recruited to form complex patterns of locomotion.


PUBLICATIONS
Bercier, V., Hubbard, J.M., Fidelin, K., Duroure, K., Auer, T.O., Revenu, C., Wyart, C., Del Bene, F. (2019) Dynactin1 depletion leads to neuromuscular synapse instability and functional abnormalities. Molecular neurodegeneration. 14:27
Desban, L., Prendergast, A., Roussel, J., Rosello, M., Geny, D., Wyart, C., Bardet, P.L. (2019) Regulation of the apical extension morphogenesis tunes the mechanosensory response of microvilliated neurons. PLoS Biology. 17:e3000235
Wyart, C., Thirumalai, V. (2019) Building behaviors, one layer at a time. eLIFE. 8
Sternberg, J.R., Prendergast, A.E., Brosse, L., Cantaut-Belarif, Y., Thouvenin, O., Orts-Del'Immagine, A., Castillo, L., Djenoune, L., Kurisu, S., McDearmid, J.R., Bardet, P.L., Boccara, C., Okamoto, H., Delmas, P., Wyart, C. (2018) Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature. Nature communications. 9:3804
Severi, K.E., Böhm, U.L., Wyart, C. (2018) Investigation of hindbrain activity during active locomotion reveals inhibitory neurons involved in sensorimotor processing. Scientific Reports. 8:13615
Cantaut-Belarif, Y., Sternberg, J.R., Thouvenin, O., Wyart, C., Bardet, P.L. (2018) The Reissner Fiber in the Cerebrospinal Fluid Controls Morphogenesis of the Body Axis. Current biology : CB. 28(15):2479-2486.e4
Knafo, S., Wyart, C. (2018) Active mechanosensory feedback during locomotion in the zebrafish spinal cord. Current opinion in neurobiology. 52:48-53
Thouvenin, O., Wyart, C. (2017) Tracking microscopy enables whole-brain imaging in freely moving zebrafish. Nature Methods. 14:1041-1042
Knafo, S., Prendergast, A., Thouvenin, O., Figueiredo, S.N., Wyart, C. (2017) Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae. Bio-protocol. 7:e2550
Djenoune, L., Wyart, C. (2017) Light on a sensory interface linking the cerebrospinal fluid to motor circuits in vertebrates. Journal of neurogenetics. 31(3):113-127
Wyart, C., Knafo, S., Fidelin, K., Prendergast, A., Tseng, P.B., Parrin, A., Dickey, C.W., Böhm, U.L., Nunes FIgueiredo, S., Thouvenin, O., Pascal-Moussellard, H. (2017) Mechanosensory neurons control the timing of spinal microcircuit selection during locomotion. eLIFE. 6
Djenoune, L., Desban, L., Gomez, J., Sternberg, J.R., Prendergast, A., Langui, D., Quan, F.B., Marnas, H., Auer, T.O., Rio, J.P., Del Bene, F., Bardet, P.L., Wyart, C. (2017) The dual developmental origin of spinal cerebrospinal fluid-contacting neurons gives rise to distinct functional subtypes. Scientific Reports. 7:719
Hubbard, J.M., Böhm, U.L., Prendergast, A., Tseng, P.B., Newman, M., Stokes, C., Wyart, C. (2016) Intraspinal Sensory Neurons Provide Powerful Inhibition to Motor Circuits Ensuring Postural Control during Locomotion. Current biology : CB. 26(21):2841-2853
Sternberg, J.R., Severi, K.E., Fidelin, K., Gomez, J., Ihara, H., Alcheikh, Y., Hubbard, J.M., Kawakami, K., Suster, M., Wyart, C. (2016) Optimization of a Neurotoxin to Investigate the Contribution of Excitatory Interneurons to Speed Modulation In Vivo. Current biology : CB. 26(17):2319-28
Hernandez, O., Papagiakoumou, E., Tanese, D., Fidelin, K., Wyart, C., Emiliani, V. (2016) Three-dimensional spatiotemporal focusing of holographic patterns. Nature communications. 7:11928
Rupprecht, P., Prendergast, A., Wyart, C., Friedrich, R.W. (2016) Remote z-scanning with a macroscopic voice coil motor for fast 3D multiphoton laser scanning microscopy. Biomedical Optics Express. 7:1656-71
Böhm, U. L., Prendergast, A., Djenoune, L., Nunes Figueiredo, S., Gomez, J., Stokes, C., Kaiser, S., Suster, M., Kawakami, K., Charpentier, M., Concordet, J. P., Rio, J. P., Del Bene, F., Wyart, C. (2016) CSF-contacting neurons regulate locomotion by relaying mechanical stimuli to spinal circuits. Nature communications. 7:10866
Fidelin, K., Djenoune, L., Stokes, C., Prendergast, A., Gomez, J., Baradel, A., Del Bene, F., Wyart, C. (2015) State-Dependent Modulation of Locomotion by GABAergic Spinal Sensory Neurons. Current biology : CB. 25:3035-47
Lauterbach, M.A., Ronzitti, E., Sternberg, J.R., Wyart, C., Emiliani, V. (2015) Fast Calcium Imaging with Optical Sectioning via HiLo Microscopy. PLoS One. 10:e0143681
Knafo, S., Wyart, C. (2015) Optogenetic neuromodulation: New tools for monitoring and breaking neural circuits. Annals of physical and rehabilitation medicine. 58(4):259-64
Auer, T.O., Xiao, T., Bercier, V., Gebhardt, C., Duroure, K., Concordet, J.P., Wyart, C., Suster, M., Kawakami, K., Wittbrodt, J., Baier, H., Del Bene, F. (2015) Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. eLIFE. 4
De Vico Fallani, F., Corazzol, M., Sternberg, J., Wyart, C., Chavez, M. (2015) Hierarchy of Neural Organization in the Embryonic Spinal Cord: Granger-Causality Graph Analysis of Calcium Imaging Data. IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society. 23(3):333-41
Quan, F.B., Dubessy, C., Galant, S., Kenigfest, N.B., Djenoune, L., Leprince, J., Wyart, C., Lihrmann, I., Tostivint, H. (2015) Comparative Distribution and In Vitro Activities of the Urotensin II-Related Peptides URP1 and URP2 in Zebrafish: Evidence for Their Colocalization in Spinal Cerebrospinal Fluid-Contacting Neurons. PLoS One. 10:e0119290
Sternberg, J. R., Wyart, C. (2015) Neuronal wiring: linking dendrite placement to synapse formation. Current biology : CB. 25(5):R190-R191
Fidelin, K., and Wyart, C. (2014) Inhibition and motor control in the developing zebrafish spinal cord. Current opinion in neurobiology. 26C:103-109
Djenoune, L., Khabou, H., Joubert, F., Quan, F.B., Nunes Figueiredo, S., Bodineau, L., Del Bene, F., Burcklé, C., Tostivint, H., Wyart, C. (2014) Investigation of spinal cerebrospinal fluid-contacting neurons expressing PKD2L1: evidence for a conserved system from fish to primates. Frontiers in Neuroanatomy. 8:26
Goetz, J.G., Steed, E., Ferreira, R.R., Roth, S., Ramspacher, C., Boselli, F., Charvin, G., Liebling, M., Wyart, C., Schwab, Y., Vermot, J. (2014) Endothelial cilia mediate low flow sensing during zebrafish vascular development. Cell Reports. 6:799-808
Hong, E., Santhakumar, K., Akitake, C.A., Ahn, S.J., Thisse, C., Thisse, B., Wyart, C., Mangin, J.M., and Halpern, M.E. (2013) Cholinergic left-right asymmetry in the habenulo-interpeduncular pathway. Proceedings of the National Academy of Sciences of the United States of America. 110(52):21171-21176
Mirat, O., Sternberg, J.R., Severi, K.E., and Wyart, C. (2013) ZebraZoom: an automated program for high-throughput behavioral analysis and categorization. Frontiers in neural circuits. 7:107
Portugues, R., Severi, K.E., Wyart, C., and Ahrens, M.B. (2013) Optogenetics in a transparent animal: circuit function in the larval zebrafish. Current opinion in neurobiology. 23(1):119-126
Del Bene, F., and Wyart, C. (2012) Optogenetics: A new enlightenment age for zebrafish neurobiology. Developmental Neurobiology. 72(3):404-414
Warp, E., Agarwal, G., Wyart, C., Friedmann, D., Oldfield, C.S., Conner, A., Del Bene, F., Arrenberg, A.B., Baier, H., and Isacoff, E.Y. (2012) Emergence of Patterned Activity in the Developing Zebrafish Spinal Cord. Current biology : CB. 22(2):93-102
Wyart, C., and Del Bene, F. (2011) Let there be light: zebrafish neurobiology and the optogenetic revolution. Reviews in the Neurosciences. 22(1):121-130
Del Bene, F., Wyart, C., Robles, E., Tran, A., Looger, L., Scott, E.K., Isacoff, E.Y., and Baier, H. (2010) Filtering of visual information in the tectum by an identified neural circuit. Science (New York, N.Y.). 330(6004):669-673
Janovjak, H., Szobota, S., Wyart, C., Trauner, D., and Isacoff, E.Y. (2010) A light-gated, potassium-selective glutamate receptor for the optical inhibition of neuronal firing. Nature Neuroscience. 13(8):1027-1032
Wyart, C., Bene, F.D., Warp, E., Scott, E.K., Trauner, D., Baier, H., and Isacoff, E.Y. (2009) Optogenetic dissection of a behavioural module in the vertebrate spinal cord. Nature. 461(7262):407-410
Szobota, S., Gorostiza, P., Del Bene, F., Wyart, C., Fortin, D.L., Kolstad, K.D., Tulyathan, O., Volgraf, M., Numano, R., Aaron, H.L., Scott, E.K., Kramer, R.H., Flannery, J., Baier, H., Trauner, D., and Isacoff, E.Y. (2007) Remote control of neuronal activity with a light-gated glutamate receptor. Neuron. 54(4):535-545

NON-ZEBRAFISH PUBLICATIONS