Ablation of EYS in zebrafish causes mislocalisation of outer segment proteins, F-actin disruption and cone-rod dystrophy

Lu, Z., Hu, X., Liu, F., Soares, D.C., Liu, X., Yu, S., Gao, M., Han, S., Qin, Y., Li, C., Jiang, T., Luo, D., Guo, A.Y., Tang, Z., Liu, M.
Scientific Reports   7: 46098 (Journal)
Registered Authors
Gao, Meng, Han, Shanshan, Hu, Xuebin, Li, Chang, Liu, Fei, Liu, Mugen, Liu, Xiliang, Lu, Zhaojing, Qin, Yayun, Yu, Shanshan
Development, Retina
MeSH Terms
  • Actins/metabolism*
  • Aging/pathology
  • Animals
  • Apoptosis
  • Base Sequence
  • Cone-Rod Dystrophies/metabolism*
  • Cone-Rod Dystrophies/pathology
  • Cone-Rod Dystrophies/physiopathology
  • Electroretinography
  • Eye Proteins/metabolism*
  • Gene Deletion*
  • Gene Knockout Techniques
  • Light Signal Transduction
  • Rod Cell Outer Segment/metabolism*
  • Rod Cell Outer Segment/pathology
  • Rod Cell Outer Segment/ultrastructure
  • Transcription Activator-Like Effector Nucleases/metabolism
  • Vision, Ocular
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
28378834 Full text @ Sci. Rep.
Mutations in EYS are associated with autosomal recessive retinitis pigmentosa (arRP) and autosomal recessive cone-rod dystrophy (arCRD) however, the function of EYS and the molecular mechanisms of how these mutations cause retinal degeneration are still unclear. Because EYS is absent in mouse and rat, and the structure of the retina differs substantially between humans and Drosophila, we utilised zebrafish as a model organism to study the function of EYS in the retina. We constructed an EYS-knockout zebrafish-line by TALEN technology which showed visual impairment at an early age, while the histological and immunofluorescence assays indicated the presence of progressive retinal degeneration with a cone predominately affected pattern. These phenotypes recapitulate the clinical manifestations of arCRD patients. Furthermore, the EYS-/- zebrafish also showed mislocalisation of certain outer segment proteins (rhodopsin, opn1lw, opn1sw1, GNB3 and PRPH2), and disruption of actin filaments in photoreceptors. Protein mislocalisation may, therefore, disrupt the function of cones and rods in these zebrafish and cause photoreceptor death. Collectively, these results point to a novel role for EYS in maintaining the morphological structure of F-actin and in protein transport, loss of this function might be the trigger for the resultant cellular events that ultimately lead to photoreceptor death.
Genes / Markers
Show all Figures
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes