PUBLICATION
Photosynthetic biomaterials: a pathway towards autotrophic tissue engineering
- Authors
- Schenck, T.L., Hopfner, U., Chávez, M.N., Machens, H.G., Somlai-Schweiger, I., Giunta, R.E., Bohne, A.V., Nickelsen, J., Allende, M.L., Egaña, J.T.
- ID
- ZDB-PUB-170214-243
- Date
- 2015
- Source
- Acta biomaterialia 15: 39-47 (Journal)
- Registered Authors
- Allende, Miguel L.
- Keywords
- Biomaterials, Chlamydomonas reinhardtii, Hypoxia, Photosynthesis, Tissue engineering
- MeSH Terms
-
- Animals
- Autotrophic Processes/drug effects*
- Biocompatible Materials/pharmacology*
- Chlamydomonas reinhardtii/drug effects
- Chlamydomonas reinhardtii/growth & development
- Female
- Implants, Experimental
- Inflammation/pathology
- Mice, Nude
- Microalgae/growth & development
- Models, Animal
- Photosynthesis/drug effects*
- Tissue Engineering/methods*
- Tissue Scaffolds/chemistry
- Zebrafish
- PubMed
- 25536030 Full text @ Acta Biomater
Citation
Schenck, T.L., Hopfner, U., Chávez, M.N., Machens, H.G., Somlai-Schweiger, I., Giunta, R.E., Bohne, A.V., Nickelsen, J., Allende, M.L., Egaña, J.T. (2015) Photosynthetic biomaterials: a pathway towards autotrophic tissue engineering. Acta biomaterialia. 15:39-47.
Abstract
Engineered tissues are highly limited by poor vascularization in vivo, leading to hypoxia. In order to overcome this challenge, we propose the use of photosynthetic biomaterials to provide oxygen. Since photosynthesis is the original source of oxygen for living organisms, we suggest that this could be a novel approach to provide a constant source of oxygen supply independently of blood perfusion. In this study we demonstrate that bioartificial scaffolds can be loaded with a solution containing the photosynthetic microalgae Chlamydomonas reinhardtii, showing high biocompatibility and photosynthetic activity in vitro. Furthermore, when photosynthetic biomaterials were engrafted in a mouse full skin defect, we observed that the presence of the microalgae did not trigger a native immune response in the host. Moreover, the analyses showed that the algae survived for at least 5 days in vivo, generating chimeric tissues comprised of algae and murine cells. The results of this study represent a crucial step towards the establishment of autotrophic tissue engineering approaches and suggest the use of photosynthetic cells to treat a broad spectrum of hypoxic conditions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping