ZFIN ID: ZDB-PUB-170206-1
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.
Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods
Varas, M., Fariña, A., Díaz-Pascual, F., Ortíz-Severín, J., Marcoleta, A.E., Allende, M.L., Santiviago, C.A., Chávez, F.P.
Date: 2017
Source: Journal of Microbiological Methods 135: 20-25 (Journal)
Registered Authors: Allende, Miguel L.
Keywords: Danio rerio, Host-pathogen interaction, Immune response, Infection, Live-cell imaging, Salmonella, Zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/immunology
  • Animals, Genetically Modified/microbiology
  • Disease Models, Animal
  • Host-Pathogen Interactions/immunology
  • Immersion
  • Immunity, Innate/immunology
  • Larva/immunology
  • Larva/microbiology*
  • Microinjections/methods*
  • Microinjections/veterinary*
  • Neutrophils/immunology
  • Salmonella Infections/diagnostic imaging*
  • Salmonella Infections/immunology
  • Salmonella Infections/microbiology*
  • Salmonella typhimurium/pathogenicity*
  • Zebrafish/immunology
  • Zebrafish/microbiology*
PubMed: 28161588 Full text @ J. Microbiol. Methods
Citation
Varas, M., Fariña, A., Díaz-Pascual, F., Ortíz-Severín, J., Marcoleta, A.E., Allende, M.L., Santiviago, C.A., Chávez, F.P. (2017) Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods. Journal of Microbiological Methods. 135:20-25.
FIGURES
ABSTRACT
The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune response induced by GFP-tagged Salmonella Typhimurium inoculated by immersion and microinjection in transgenic zebrafish larvae. Our novel infection protocols in zebrafish allow live-cell imaging of Salmonella colonization.
ADDITIONAL INFORMATIONNo data available