PUBLICATION
Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods
- Authors
- Varas, M., Fariña, A., Díaz-Pascual, F., Ortíz-Severín, J., Marcoleta, A.E., Allende, M.L., Santiviago, C.A., Chávez, F.P.
- ID
- ZDB-PUB-170206-1
- Date
- 2017
- Source
- Journal of Microbiological Methods 135: 20-25 (Journal)
- Registered Authors
- Allende, Miguel L.
- Keywords
- Danio rerio, Host-pathogen interaction, Immune response, Infection, Live-cell imaging, Salmonella, Zebrafish
- MeSH Terms
-
- Salmonella typhimurium/pathogenicity*
- Zebrafish/immunology
- Zebrafish/microbiology*
- Neutrophils/immunology
- Disease Models, Animal
- Larva/immunology
- Larva/microbiology*
- Animals, Genetically Modified/immunology
- Animals, Genetically Modified/microbiology
- Host-Pathogen Interactions/immunology
- Animals
- Immunity, Innate/immunology
- Microinjections/methods*
- Microinjections/veterinary*
- Immersion
- Salmonella Infections/diagnostic imaging*
- Salmonella Infections/immunology
- Salmonella Infections/microbiology*
- PubMed
- 28161588 Full text @ J. Microbiol. Methods
Citation
Varas, M., Fariña, A., Díaz-Pascual, F., Ortíz-Severín, J., Marcoleta, A.E., Allende, M.L., Santiviago, C.A., Chávez, F.P. (2017) Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods. Journal of Microbiological Methods. 135:20-25.
Abstract
The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune response induced by GFP-tagged Salmonella Typhimurium inoculated by immersion and microinjection in transgenic zebrafish larvae. Our novel infection protocols in zebrafish allow live-cell imaging of Salmonella colonization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping