ZFIN ID: ZDB-PUB-170206-1
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Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods
Varas, M., Fariña, A., Díaz-Pascual, F., Ortíz-Severín, J., Marcoleta, A.E., Allende, M.L., Santiviago, C.A., Chávez, F.P.
Date: 2017
Source: Journal of Microbiological Methods   135: 20-25 (Journal)
Registered Authors: Allende, Miguel L.
Keywords: Danio rerio, Host-pathogen interaction, Immune response, Infection, Live-cell imaging, Salmonella, Zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/immunology
  • Animals, Genetically Modified/microbiology
  • Disease Models, Animal
  • Host-Pathogen Interactions/immunology
  • Immersion
  • Immunity, Innate/immunology
  • Larva/immunology
  • Larva/microbiology*
  • Microinjections/methods*
  • Microinjections/veterinary*
  • Neutrophils/immunology
  • Salmonella Infections/diagnostic imaging*
  • Salmonella Infections/immunology
  • Salmonella Infections/microbiology*
  • Salmonella typhimurium/pathogenicity*
  • Zebrafish/immunology
  • Zebrafish/microbiology*
PubMed: 28161588 Full text @ J. Microbiol. Methods
Citation
Varas, M., Fariña, A., Díaz-Pascual, F., Ortíz-Severín, J., Marcoleta, A.E., Allende, M.L., Santiviago, C.A., Chávez, F.P. (2017) Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods. Journal of Microbiological Methods. 135:20-25.
FIGURES
ABSTRACT
The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune response induced by GFP-tagged Salmonella Typhimurium inoculated by immersion and microinjection in transgenic zebrafish larvae. Our novel infection protocols in zebrafish allow live-cell imaging of Salmonella colonization.
ADDITIONAL INFORMATION