ZFIN ID: ZDB-PUB-170206-1
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Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods
Varas, M., Fariña, A., Díaz-Pascual, F., Ortíz-Severín, J., Marcoleta, A.E., Allende, M.L., Santiviago, C.A., Chávez, F.P.
Date: 2017
Source: Journal of Microbiological Methods 135: 20-25 (Journal)
Registered Authors: Allende, Miguel L.
Keywords: Danio rerio, Host-pathogen interaction, Immune response, Infection, Live-cell imaging, Salmonella, Zebrafish
MeSH Terms: Animals; Animals, Genetically Modified/immunology; Animals, Genetically Modified/microbiology; Disease Models, Animal; Host-Pathogen Interactions/immunology (all 18) expand
PubMed: 28161588 Full text @ J. Microbiol. Methods
Citation
Varas, M., Fariña, A., Díaz-Pascual, F., Ortíz-Severín, J., Marcoleta, A.E., Allende, M.L., Santiviago, C.A., Chávez, F.P. (2017) Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods. Journal of Microbiological Methods. 135:20-25.
FIGURES   (current status)
ABSTRACT
The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune response induced by GFP-tagged Salmonella Typhimurium inoculated by immersion and microinjection in transgenic zebrafish larvae. Our novel infection protocols in zebrafish allow live-cell imaging of Salmonella colonization.
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