PUBLICATION

Studying the adaptive immune system in zebrafish by transplantation of hematopoietic precursor cells

Authors
Iwanami, N., Hess, I., Schorpp, M., Boehm, T.
ID
ZDB-PUB-170129-25
Date
2017
Source
Methods in cell biology   138: 151-161 (Chapter)
Registered Authors
Boehm, Tom, Hess, Isabell, Schorpp, Michael
Keywords
Hematopoiesis, Thymus, Transplantation, cmyb
MeSH Terms
  • Adaptive Immunity/genetics
  • Adaptive Immunity/immunology*
  • Animals
  • Embryo, Nonmammalian
  • Hematopoiesis/genetics
  • Hematopoietic Stem Cell Transplantation/methods*
  • Hematopoietic Stem Cells/immunology
  • Molecular Biology/methods*
  • Mutation
  • Proto-Oncogene Proteins c-myb/genetics
  • Proto-Oncogene Proteins c-myb/immunology
  • Zebrafish/genetics
  • Zebrafish/immunology*
PubMed
28129842 Full text @ Meth. Cell. Biol.
Abstract
Traditionally, transplantation has been a major experimental procedure to study the development and function of hematopoietic and immune systems. Here, we describe the use of a zebrafish strain lacking definitive hematopoiesis (cmybI181N) for interspecific analysis of hematopoietic and immune cell development. Without conditioning prior to transplantation, allogeneic and xenogeneic hematopoietic progenitor cells stably engraft in adult zebrafish homozygous for the cmyb mutation. This unique animal model can be used to genetically and functionally disentangle universal and species-specific contributions of the microenvironment to hematopoietic progenitor cell maintenance and development.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes