PUBLICATION
Studying the adaptive immune system in zebrafish by transplantation of hematopoietic precursor cells
- Authors
- Iwanami, N., Hess, I., Schorpp, M., Boehm, T.
- ID
- ZDB-PUB-170129-25
- Date
- 2017
- Source
- Methods in cell biology 138: 151-161 (Chapter)
- Registered Authors
- Boehm, Tom, Hess, Isabell, Schorpp, Michael
- Keywords
- Hematopoiesis, Thymus, Transplantation, cmyb
- MeSH Terms
-
- Adaptive Immunity/genetics
- Adaptive Immunity/immunology*
- Animals
- Embryo, Nonmammalian
- Hematopoiesis/genetics
- Hematopoietic Stem Cell Transplantation/methods*
- Hematopoietic Stem Cells/immunology
- Molecular Biology/methods*
- Mutation
- Proto-Oncogene Proteins c-myb/genetics
- Proto-Oncogene Proteins c-myb/immunology
- Zebrafish/genetics
- Zebrafish/immunology*
- PubMed
- 28129842 Full text @ Meth. Cell. Biol.
Citation
Iwanami, N., Hess, I., Schorpp, M., Boehm, T. (2017) Studying the adaptive immune system in zebrafish by transplantation of hematopoietic precursor cells. Methods in cell biology. 138:151-161.
Abstract
Traditionally, transplantation has been a major experimental procedure to study the development and function of hematopoietic and immune systems. Here, we describe the use of a zebrafish strain lacking definitive hematopoiesis (cmybI181N) for interspecific analysis of hematopoietic and immune cell development. Without conditioning prior to transplantation, allogeneic and xenogeneic hematopoietic progenitor cells stably engraft in adult zebrafish homozygous for the cmyb mutation. This unique animal model can be used to genetically and functionally disentangle universal and species-specific contributions of the microenvironment to hematopoietic progenitor cell maintenance and development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping