ZFIN ID: ZDB-PUB-160916-3
A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease
Don, E.K., Formella, I., Badrock, A.P., Hall, T.E., Morsch, M., Hortle, E., Hogan, A., Chow, S., Gwee, S.S., Stoddart, J.J., Nicholson, G., Chung, R., Cole, N.J.
Date: 2017
Source: Zebrafish   14(1): 69-72 (Journal)
Registered Authors: Badrock, Andrew P., Cole, Nicholas, Don, Emily, Hall, Thomas, Stoddart, Jack
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/genetics*
  • DNA Transposable Elements*
  • DNA, Recombinant/genetics
  • Gene Transfer Techniques*
  • Genetic Vectors
  • Green Fluorescent Proteins/genetics
  • Nervous System Diseases/genetics*
  • Neurodegenerative Diseases/genetics*
  • Promoter Regions, Genetic
  • Recombination, Genetic
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed: 27631880 Full text @ Zebrafish
Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover effective therapeutics. We have generated a Tol2 Gateway-compatible toolbox to study neurodegenerative disorders in zebrafish, which includes promoters for astrocytes, microglia and motor neurons, multiple fluorophores, and compatibility for the introduction of genes of interest or disease-linked genes. This toolbox will advance the rapid and flexible generation of zebrafish models to discover the biology of the nervous system and the disease processes that lead to neurodegeneration.