ZFIN ID: ZDB-PUB-160729-20
Melanoma Regression and Recurrence in Zebrafish
Wojciechowska, S., Zeng, Z., Lister, J.A., Ceol, C.J., Patton, E.E.
Date: 2016
Source: Methods in molecular biology (Clifton, N.J.)   1451: 143-53 (Chapter)
Registered Authors: Ceol, Craig, Lister, James A., Patton, E. Elizabeth, Zeng, Zhiqiang
Keywords: Melanoma, Mitf, Recurrence, Regression, Zebrafish cancer models
MeSH Terms:
  • Animals
  • Humans
  • Immunohistochemistry
  • Melanoma/genetics
  • Melanoma/metabolism
  • Melanoma/pathology*
  • Microphthalmia-Associated Transcription Factor/genetics
  • Microphthalmia-Associated Transcription Factor/metabolism
  • Neoplasms/genetics
  • Neoplasms/pathology*
  • Proto-Oncogene Proteins B-raf/genetics
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 27464806 Full text @ Meth. Mol. Biol.
Melanoma is the most lethal form of skin cancer with high mortality rates. Most melanoma cases have activating mutations in BRAF (V600E) and the selective inhibitors of BRAF(V600E) have been successfully used in patients. However, after initial tumor regression, the majority of patients develop drug resistance resulting in tumor regrowth. It is therefore important to understand the mechanisms underlying these processes. We have recently described the role of the master melanocyte transcription factor MITF in tumor growth, regression, and recurrence. Here, we describe protocols to study regression and recurrence in vivo, as well as for histology and immunohistochemistry, using a temperature-sensitive zebrafish model of human melanoma.