|ZFIN ID: ZDB-PUB-160729-14|
Studying Lipid Metabolism and Transport During Zebrafish Development
Zeituni, E.M., Farber, S.A.
|Source:||Methods in molecular biology (Clifton, N.J.) 1451: 237-55 (Chapter)|
|Registered Authors:||Farber, Steven|
|Keywords:||Lipid, Metabolism, Microscopy, Thin layer chromatography, Transport, Zebrafish|
|PubMed:||27464812 Full text @ Meth. Mol. Biol.|
Zeituni, E.M., Farber, S.A. (2016) Studying Lipid Metabolism and Transport During Zebrafish Development. Methods in molecular biology (Clifton, N.J.). 1451:237-55.
ABSTRACTThe zebrafish model facilitates the study of lipid metabolism and transport during development. Here, we outline methods to introduce traceable fluorescent or radiolabeled fatty acids into zebrafish embryos and larvae at various developmental stages. Labeled fatty acids can be injected into the large yolk cell prior to the development of digestive organs when the larvae is entirely dependent on the yolk for its nutrition (lecithotrophic state). Once zebrafish are able to consume exogenous food, labeled fatty acids can be incorporated into their food. Our group and others have demonstrated that the transport and processing of these injected or ingested fatty acid analogs can be followed through microscopy and/or biochemical analysis. These techniques can be easily combined with targeted antisense approaches, transgenics, or drug treatments (see Note 1 ), allowing studies of lipid cell biology and metabolism that are exceedingly difficult or impossible in mammals.
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