PUBLICATION

Roles of Wnt pathway genes wls, wnt9a, wnt5b, frzb and gpc4 in regulating convergent-extension during zebrafish palate morphogenesis

Authors
Rochard, L., Monica, S.D., Ling, I.T., Kong, Y., Roberson, S., Harland, R., Halpern, M., Liao, E.C.
ID
ZDB-PUB-160722-12
Date
2016
Source
Development (Cambridge, England)   143: 2541-7 (Journal)
Registered Authors
Halpern, Marnie E., Harland, Richard, Liao, Eric, Roberson, Sara
Keywords
Convergent-extension, Morphogenesis, Palate, Wnt, Zebrabow
MeSH Terms
  • Animals
  • Cell Proliferation
  • Cell Shape
  • Chondrocytes/metabolism
  • Epistasis, Genetic
  • Morphogenesis/genetics*
  • Mutation/genetics
  • Palate/embryology*
  • Palate/metabolism*
  • Phenotype
  • Wnt Signaling Pathway/genetics*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
27287801 Full text @ Development
Abstract
The Wnt signaling pathway is crucial for tissue morphogenesis, participating in cellular behavior changes, notably during the process of convergent-extension. Interactions between Wnt-secreting and receiving cells during convergent-extension remain elusive. We investigated the role and genetic interactions of Wnt ligands and their trafficking factors Wls, Gpc4 and Frzb in the context of palate morphogenesis in zebrafish. We describe that the chaperon Wls and its ligands Wnt9a and Wnt5b are expressed in the ectoderm, whereas juxtaposed chondrocytes express Frzb and Gpc4. Using wls, gpc4, frzb, wnt9a and wnt5b mutants, we genetically dissected the Wnt signals operating between secreting ectoderm and receiving chondrocytes. Our analysis delineates that non-canonical Wnt signaling is required for cell intercalation, and that wnt5b and wnt9a are required for palate extension in the anteroposterior and transverse axes, respectively.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes