PUBLICATION

CD271 downregulation promotes melanoma progression and invasion in 3-dimensional models and in zebrafish

Authors
Saltari, A., Truzzi, F., Quadri, M., Lotti, R., Palazzo, E., Grisendi, G., Tiso, N., Marconi, A., Pincelli, C.
ID
ZDB-PUB-160622-2
Date
2016
Source
The Journal of investigative dermatology   136(10): 2049-58 (Journal)
Registered Authors
Tiso, Natascia
Keywords
none
MeSH Terms
  • Animals
  • Cell Adhesion
  • Cell Line, Tumor
  • Disease Progression
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic/genetics*
  • Gene Silencing
  • Humans
  • Melanoma/genetics
  • Melanoma/pathology*
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • Neoplasm Staging
  • Nerve Tissue Proteins/genetics*
  • Receptors, Nerve Growth Factor/genetics*
  • Skin Neoplasms/genetics
  • Skin Neoplasms/pathology*
  • Zebrafish
PubMed
27328305 Full text @ J. Invest. Dermatol.
Abstract
CD271 is a neurotrophin receptor variably expressed in melanoma. While contradictory data are reported on its role as a marker of tumor initiating cells, little is known on its function in tumor progression. CD271 expression was higher in spheroids derived from freshly isolated cells of primary melanomas and in primary WM115 and WM793-B cell lines, while it decreased during progression to advanced stages in cells isolated from metastatic melanomas and in metastatic WM266-4 and 1205Lu cell lines. Moreover, CD271 was scarcely detected in the highly invasive spheroids (SKMEL28 and 1205Lu). CD271, originally expressed in the epidermis of skin reconstructs, disappeared when melanoma started to invade the dermis. SKMEL8 CD271(-) cells showed greater proliferation and invasiveness in vitro, and were associated with a higher number of metastases in zebrafish, as compared to CD271(+) cells. CD271 silencing in WM115 induced a more aggressive phenotype in vitro and in vivo. On the contrary, CD271 overexpression in SKMEL28 cells reduced invasion in vitro, and CD271 overexpressing 1205Lu cells was associated with a lower percentage of metastases in zebrafish. A reduced cell-cell adhesion was also observed in absence of CD271. Taken together, these results indicate that CD271 loss is critical for melanoma progression and metastasis.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes