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Research
General Information
ZIRC
ZFIN ID: ZDB-PERS-020116-8
Tiso, Natascia
Email: natascia.tiso@unipd.it
URL: http://www.bio.unipd.it/development/UNIT/HOME.html
Affiliation: Tiso Lab
Address: Developmental Biology Lab Department of Biology University of Padova via U. Bassi 58/B Padova, I-35131 Italy
Country: Italy
Phone: +39-049-827-6302
Fax:
Orcid ID: 0000-0002-5444-9853


BIOGRAPHY AND RESEARCH INTERESTS
Personal data
Nationality: Italian
Foreign languages: English, French.

Qualifications
July 1989: School-leaving Certificate (Scientific High School 'E. Fermi', Padova); full marks (60 / 60).
13th of July 1994: Graduation on Biological Sciences (Biomolecular Course), University of Padova;
first-class honours degree (110 / 110 magna cum laude).
January 1996: Official State Qualification as a Professional Biologist, University of Padova.
15th of January - 20th of December 1997: Visiting Scientist at the Human Genetics Laboratory (Supervisor: Dr. J. M. Rommens - discoverer of the CF gene), Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
May 1998: PhD in Genetics, Universities of Bologna, Ferrara and Padova.
September 1998: Two-year Post-doctoral Fellowship at the Human Genetics Laboratory, University of Padova.
December 2000: Three-year EU contract (Research Assistant) at the Laboratory of Developmental Biology, University of Padova.
March 2004: Four-year EU contract (Research Assistant) in the above-mentioned work place.
Aug-Dec 2005: EU-Screen at the Max Planck Institute of Tuebingen - Germany (Supervisor: Prof. C. Nuesslein-Volhard - Nobel Laureate).
October 2007-August 2015: Assistant Professor in Genetics, University of Padova, Italy.
September 2015-present: Associate Professor in Applied Biology, University of Padova, Italy

Research activity
My research activity dealt with the localisation and the characterisation of human disease genes by linkage analysis, positional cloning, mutation screening and protein interaction studies. In particular, I worked on one form of Arrhithmogenic Right Ventricular Cardiomyopathy, whose locus (ARVD2) has been previously mapped in Dr Danieli's laboratory, and I identified the causative gene (RYR2).
My work experience also included systematic mapping of ESTs and human genes by monochromosomal and radiation hybrid panels; cDNA selection from specific sub-chromosomal regions and reconstruction of the genomic structure of human genes by Long-Distance, Alu and in silico PCR.
My present research interests mainly concern signaling pathway analysis and disease modeling in zebrafish, with particular attention to the neural, pancreatic, thyroidal, cardiac and mitochondrial compartment.
These studies are carried out by mRNA and morpholino microinjection, microarray strategies, single and double whole mount in situ hybridisation in zebrafish embryos, xeno-transplantations, Crispr/Cas9 genome editing, analysis of mutants and production of pathway reporter transgenic lines.


PUBLICATIONS
Deiana, M., Dalle Carbonare, L., Serena, M., Cheri, S., Parolini, F., Gandini, A., Marchetto, G., Innamorati, G., Manfredi, M., Marengo, E., Brandi, J., Cecconi, D., Mori, A., Mina, M.M., Antoniazzi, F., Mottes, M., Tiso, N., Malerba, G., Zipeto, D., Valenti, M.T. (2018) New Insights into the Runt Domain of RUNX2 in Melanoma Cell Proliferation and Migration. Cells. 7(11)
Facchinello, N., Skobo, T., Meneghetti, G., Colletti, E., Dinarello, A., Tiso, N., Costa, R., Gioacchini, G., Carnevali, O., Argenton, F., Colombo, L., Valle, L.D. (2018) Author Correction: nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor. Scientific Reports. 8:4445
Giuliodori, A., Beffagna, G., Marchetto, G., Fornetto, C., Vanzi, F., Toppo, S., Facchinello, N., Santimaria, M., Vettori, A., Rizzo, S., Della Barbera, M., Pilichou, K., Argenton, F., Thiene, G., Tiso, N., Basso, C. (2018) Loss of cardiac Wnt/β-catenin signalling in Desmoplakin-deficient AC8 zebrafish models is rescuable by genetic and pharmacological intervention. Cardiovascular research. 114(8):1082-1097
Facchinello, N., Tarifeño-Saldivia, E., Grisan, E., Schiavone, M., Peron, M., Mongera, A., Ek, O., Schmitner, N., Meyer, D., Peers, B., Tiso, N., Argenton, F. (2017) Tcf7l2 plays pleiotropic roles in the control of glucose homeostasis, pancreas morphology, vascularization and regeneration. Scientific Reports. 7:9605
Ghosal, S., Banerjee, S., Tiso, N., Grisan, E., Chowdhury, A.S. (2017) A novel non-rigid registration algorithm for zebrafish larval images. Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference. 2017:321-324
Facchinello, N., Skobo, T., Meneghetti, G., Colletti, E., Dinarello, A., Tiso, N., Costa, R., Gioacchini, G., Carnevali, O., Argenton, F., Colombo, L., Dalla Valle, L. (2017) nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor. Scientific Reports. 7:4371
Turrini, L., Fornetto, C., Marchetto, G., Müllenbroich, M.C., Tiso, N., Vettori, A., Resta, F., Masi, A., Mannaioni, G., Pavone, F.S., Vanzi, F. (2017) Optical mapping of neuronal activity during seizures in zebrafish. Scientific Reports. 7:3025
Delfino-Machín, M., Madelaine, R., Busolin, G., Nikaido, M., Colanesi, S., Camargo-Sosa, K., Law, E.W., Toppo, S., Blader, P., Tiso, N., Kelsh, R.N. (2017) Sox10 contributes to the balance of fate choice in dorsal root ganglion progenitors. PLoS One. 12:e0172947
Khatri, D., Zizioli, D., Tiso, N., Facchinello, N., Vezzoli, S., Gianoncelli, A., Memo, M., Monti, E., Borsani, G., Finazzi, D. (2016) Down-regulation of coasy, the gene associated with NBIA-VI, reduces Bmp signaling, perturbs dorso-ventral patterning and alters neuronal development in zebrafish. Scientific Reports. 6:37660
Saltari, A., Truzzi, F., Quadri, M., Lotti, R., Palazzo, E., Grisendi, G., Tiso, N., Marconi, A., Pincelli, C. (2016) CD271 downregulation promotes melanoma progression and invasion in 3-dimensional models and in zebrafish. The Journal of investigative dermatology. 136(10):2049-58
de Filippis, T., Marelli, F., Nebbia, G., Porazzi, P., Corbetta, S., Fugazzola, L., Gastaldi, R., Vigone, M.C., Biffanti, R., Frizziero, D., Mandarà, L., Prontera, P., Salerno, M., Maghnie, M., Tiso, N., Radetti, G., Weber, G., Persani, L. (2016) JAG1 loss-of-function variations as a novel predisposing event in the pathogenesis of congenital thyroid defects. The Journal of clinical endocrinology and metabolism. 101(3):861-70
Zizioli, D., Tiso, N., Guglielmi, A., Saraceno, C., Busolin, G., Giuliani, R., Khatri, D., Monti, E., Borsani, G., Argenton, F., Finazzi, D. (2016) Knock-down of pantothenate kinase 2 severely affects the development of the nervous and vascular system in zebrafish, providing new insights into PKAN disease. Neurobiology of disease. 85:35-48
Facchinello, N., Schiavone, M., Vettori, A., Argenton, F., Tiso, N. (2016) Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors. Methods in molecular biology (Clifton, N.J.). 1481:81-94
Astone, M., Pizzi, M., Peron, M., Domenichini, A., Guzzardo, V., Töchterle, S., Tiso, N., Rugge, M., Meyer, D., Argenton, F., Vettori, A. (2015) A GFP-Tagged Gross Deletion on Chromosome 1 Causes Malignant Peripheral Nerve Sheath Tumors and Carcinomas in Zebrafish. PLoS One. 10:e0145178
Hen, G., Nicenboim, J., Mayseless, O., Asaf, L., Shin, M., Busolin, G., Hofi, R., Almog, G., Tiso, N., Lawson, N.D., Yaniv, K. (2015) Venous-derived angioblasts generate organ-specific vessels during embryonic development. Development (Cambridge, England). 142(24):4266-78
Casari, A., Schiavone, M., Facchinello, N., Vettori, A., Meyer, D., Tiso, N., Moro, E., Argenton, F. (2014) A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development. Developmental Biology. 396(1):81-93
Schiavone, M., Rampazzo, E., Casari, A., Battilana, G., Persano, L., Moro, E., Liu, S., Leach, S.D., Tiso, N., Argenton, F. (2014) Zebrafish reporter lines reveal in vivo signaling pathway activities involved in pancreatic cancer. Disease models & mechanisms. 7(7):883-94
Moro, E., Vettori, A., Porazzi, P., Schiavone, M., Rampazzo, E., Casari, A., Ek, O., Facchinello, N., Astone, M., Zancan, I., Milanetto, M., Tiso, N., and Argenton, F. (2013) Generation and application of signaling pathway reporter lines in zebrafish. Molecular genetics and genomics : MGG. 288(5-6):231-242
Cavodeassi, F., Bene, F.D., Fürthauer, M., Grabher, C., Herzog, W., Lehtonen, S., Linker, C., Mercader, N., Mikut, R., Norton, W., Strähle, U., Tiso, N., and Foulkes, N.S. (2013) Report of the Second European Zebrafish Principal Investigator Meeting in Karlsruhe, Germany, March 21-24, 2012. Zebrafish. 10(1):119-23
Rampazzo, E., Persano, L., Pistollato, F., Moro, E., Frasson, C., Porazzi, P., Della Puppa, A., Bresolin, S., Battilana, G., Indraccolo, S., Te Kronnie, G., Argenton, F., Tiso, N., and Basso, G. (2013) Wnt activation promotes neuronal differentiation of Glioblastoma. Cell Death & Disease. 4:e500
Porazzi, P., Marelli, F., Benato, F., de Filippis, T., Calebiro, D., Argenton, F., Tiso, N., and Persani, L. (2012) Disruptions of Global and Jagged1-Mediated Notch Signaling Affect Thyroid Morphogenesis in the Zebrafish. Endocrinology. 153(11):5645-5658
Moro, E., Özhan, G., Mongera, A., Beis, D., Wierzbicki, C., Young, R.M., Bournele, D., Domenichini, A., Valdivia, L.E., Lum, L., Chen, C., Amatruda, J.F., Tiso, N., Weidinger, G., and Argenton, F. (2012) In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains. Developmental Biology. 366(2):327-340
Robinson, B.W., Germano, G., Song, Y., Abrams, J., Scott, M., Guariento, I., Tiso, N., Argenton, F., Basso, G., Rhodes, J., Kanki, J.P., Look, A.T., Balice-Gordon, R.J., and Felix, C.A. (2011) mll ortholog containing functional domains of human MLL is expressed throughout the zebrafish lifespan and in haematopoietic tissues. British journal of haematology. 152(3):307-321
Vettori, A., Bergamin, G., Moro, E., Vazza, G., Polo, G., Tiso, N., Argenton, F., and Mostacciuolo, M.L. (2011) Developmental defects and neuromuscular alterations due to mitofusin 2 gene (MFN2) silencing in zebrafish: A new model for Charcot-Marie-Tooth type 2A neuropathy. Neuromuscular disorders : NMD. 21(1):58-67
Vaccari, E., Deflorian, G., Bernardi, E., Pauls, S., Tiso, N., Bortolussi, M., and Argenton, F. (2010) prep1.2 and aldh1a2 participate to a positive loop required for branchial arches development in zebrafish. Developmental Biology. 343(1-2):94-103
Moro, E., Tomanin, R., Friso, A., Modena, N., Tiso, N., Scarpa, M., and Argenton, F. (2010) A novel functional role of iduronate-2-sulfatase in zebrafish early development. Matrix biology : journal of the International Society for Matrix Biology. 29(1):43-50
Porazzi, P., Calebiro, D., Benato, F., Tiso, N., and Persani, L. (2009) Thyroid gland development and function in the zebrafish model. Molecular and Cellular Endocrinology. 312(1-2):14-23
Tiso, N., Moro, E., and Argenton, F. (2009) Zebrafish Pancreas Development. Molecular and Cellular Endocrinology. 312(1-2):24-30
Tiso, N., Filippi, A., Benato, F., Negrisolo, E., Modena, N., Vaccari, E., Driever, W., and Argenton, F. (2009) Differential expression and regulation of olig genes in zebrafish. The Journal of comparative neurology. 515(3):378-396
Milanetto, M., Tiso, N., Braghetta, P., Volpin, D., Argenton, F., and Bonaldo, P. (2008) Emilin genes are duplicated and dynamically expressed during zebrafish embryonic development. Developmental dynamics : an official publication of the American Association of Anatomists. 237(1):222-232
Manzoni, M., Colombi, P., Papini, N., Rubaga, L., Tiso, N., Preti, A., Venerando, B., Tettamanti, G., Bresciani, R., Argenton, F., Borsani, G., and Monti, E. (2007) Molecular cloning and biochemical characterization of sialidases from zebrafish (Danio rerio). The Biochemical journal. 408(3):395-406
Pauls, S., Zecchin, E., Tiso, N., Bortolussi, M., and Argenton, F. (2007) Function and regulation of zebrafish nkx2.2a during development of pancreatic islet and ducts. Developmental Biology. 304(2):875-890
Zecchin, E., Filippi, A., Biemar, F., Tiso, N., Pauls, S., Ellertsdottir, E., Gnugge, L., Bortolussi, M., Driever, W., and Argenton, F. (2007) Distinct delta and jagged genes control sequential segregation of pancreatic cell types from precursor pools in zebrafish. Developmental Biology. 301(1):192-204
Romano, A., Kottra, G., Barca, A., Tiso, N., Maffia, M., Argenton, F., Daniel, H., Storelli, C., and Verri, T. (2006) The high-affinity peptide transporter PEPT2 (SLC15A2) of the zebrafish Danio rerio: functional properties, genomic organization and expression analysis. Physiological Genomics. 24(3):207-217
Zecchin, E., Conigliaro, A., Tiso, N., Argenton, F., and Bortolussi, M. (2005) Expression analysis of jagged genes in zebrafish embryos. Developmental dynamics : an official publication of the American Association of Anatomists. 233(2):638-645
Filippi, A., Tiso, N., Deflorian, G., Zecchin, E., Bortolussi, M., and Argenton, F. (2005) The basic helix-loop-helix olig3 establishes the neural plate boundary of the trunk and is necessary for development of the dorsal spinal cord. Proc. Natl. Acad. Sci. USA. 102(12):4377-4382
Zecchin, E., Mavropoulos, A., Devos, N., Filippi, A., Tiso, N., Meyer, D., Peers, B., Bortolussi, M., Argenton, F. (2004) Evolutionary conserved role of ptf1a in the specification of exocrine pancreatic fates. Developmental Biology. 268(1):174-184
Deflorian, G., Tiso, N., Ferretti, E., Meyer, D., Blasi, F., Bortolussi, M., and Argenton, F. (2004) Prep1.1 has essential genetic functions in hindbrain development and cranial neural crest cell differentiation. Development (Cambridge, England). 131(3):613-627
Verri, T., Kottra, G., Romano, A., Tiso, N., Peric, M., Maffia, M., Boll, M., Argenton, F., Daniel, H., and Storelli, C. (2003) Molecular and functional characterisation of the zebrafish (Danio rerio) PEPT1-type peptide transporter. FEBS letters. 549(1-3):115-122
Tiso, N., Filippi, A., Pauls, S., Bortolussi, M., and Argenton, F. (2002) BMP signalling regulates anteroposterior endoderm patterning in zebrafish. Mechanisms of Development. 118(1-2):29-37

NON-ZEBRAFISH PUBLICATIONS
Tiso N., Salamon M., Bagattin A., Danieli G.A., Argenton F., Bortolussi M.: 'The binding of the RyR2 calcium channel to its gating protein FKBP12.6 is oppositely affected by ARVD2 and VTSIP mutations'. Biochem. Biophys. Res. Commun. 299: 594-598 (2002).

Bauce B., Rampazzo A., Basso C., Bagattin A., Daliento L., Tiso N., Turrini P., Thiene G., Danieli G.A. and Nava A.: 'Screening for RyR2 mutations in families with effort-induced polymorphic ventricular arrhythmias and sudden death: early diagnosis of asymptomatic carriers'. J. Am. Coll. Cardiol 40(2): 341-349 (2002).

De Leo D., Turrina S., Marigo M., Tiso N., Danieli G.A.: 'Italian population data for D1S1656, D3S1358, D8S1132, D10S2325, VWA, FES/FPS, and F13A01'. Forensic Sci Int. Nov 15; 123(1):71-73 (2001).

Turrina S., De Leo D., Marigo M., Tiso N., Danieli G.A.: 'Allele frequency distributions for D1S1656, D8S1132, D10S2325, D18S51, and D21S11 loci in a north Italy population'. J. Forensic Sci. Jan 46(1): 191-192 (2001).

Tiso N., Stephan D.A., Nava A., Bagattin A., Devaney J., Stanchi F., Larderet G., Brahmbhatt B., Brown K., Bauce B., Muriago M., Basso C., Thiene G., Danieli G.A. and Rampazzo A.: 'Identification of mutations in the cardiac Ryanodine Receptor gene in families affected with arrhythmogenic right ventricular cardiomyopathy type 2 (ARVD2)'. Human Molecular Genetics, Feb 1; 10(3):189-194 (2001).

Laitinen P.J., Brown K.M., Piippo K., Swan H., Devaney J.M., Brahmbhatt B., Donarum E.A., Marino M., Tiso N., Viitasalo M., Toivonen L., Stephan D.A., Kontula K.: 'Mutations of the Cardiac Ryanodine Receptor (RyR2) Gene in Familial Polymorphic Ventricular Tachycardia'. Circulation, Jan 30; 103(4): 485-490 (2001).

Priori S.G., Napolitano C., Tiso N., Memmi M., Vignati G., Bloise R., Sorrentino V., Danieli G.A.: 'Mutations in the Cardiac Ryanodine Receptor Gene (hRyR2) underlie catecholaminergic Polymorphic Ventricular Tachycardia'. Circulation, 102: r49-r53 (2000); Circulation, Jan 30, 103(4): 196-200 (2001).

Rampazzo A., Pivotto F., Occhi G., Tiso N., Bortoluzzi S., Rowen L., Hood L., Nava A., and Danieli G.A.: 'Characterization of C14orf4, a novel intronless human gene containing a polyglutamine repeat, mapped to the ARVD1 critical region'. Biochem. Biophys. Res. Commun. Nov 30; 278(3):766-774 (2000).

Stanchi F, Corso V, Scannapieco P, Ievolella C, Negrisolo E, Tiso N, Lanfranchi G, Valle G.: 'TUBA8: A New Tissue-Specific Isoform of alpha-Tubulin That Is Highly Conserved in Human and Mouse'. Biochem. Biophys. Res. Commun. Apr 21; 270(3):1111-1118 (2000).

Rampazzo A., Nava A., Miorin M., Tiso N., Thiene G., Danieli G.A.: 'Molecular genetics of arrhythmogenic right ventricular cardiomyopathy' in From Molecule to Men (Edited by M. Zehender, G. Breithardt, H. Just Steinkopff Darmstadt), pp. 77-80 (2000).

Tiso N., Majetti M., Stanchi F., Rampazzo A., Zimbello,R., Nava A. and Danieli G.A.: 'Fine mapping and genomic structure of ACTN2, the human gene coding for the sarcomeric isoform of alpha-Actinin-2, expressed in skeletal and cardiac muscle'. Biochem. Biophys. Res. Commun. 265, 256-259 (1999).

Bortoluzzi S., Rampoldi L., Simionati B., Zimbello R., Barbon A., d'Alessi F., Tiso N., Pallavicini A., Toppo S., Cannata F., Valle G., Lanfranchi G. and Danieli G.A.: 'A comprehensive high resolution genomic transcript map of human skeletal muscle'. Genome Research, Aug., Vol 8, n. 8: 817-825 (1998).

Rampazzo A., Nava A., Miorin M., Fonderico P., Pope B., Tiso N., Livolsi B., Thiene G.and Danieli G.A: 'ARVD4, a new locus for arrhythmogenic right ventricular cardiomyopathy, maps to chromosome 2 long arm'. Genomics, Oct 15; 45(2) 259-263 (1997).

Rampoldi L., Zimbello R., Tiso N., Pallavicini A., Valle G., Lanfranchi G. and Danieli G.A.: 'Chromosomal localization of four MAPK signalling cascade genes: MEK1, MEK3, MEK4 and MEKK5'. Cytogenetics and Cell Genetics 78: 301-303 (1997).

Valle G., Faulkner G., De Antoni A., Pacchioni B., Pallavicini A., Pandolfo D., Tiso N., Toppo S., Trevisan S., Lanfranchi G.: 'Telethonin, a novel sarcomeric protein of heart and skeletal muscle'. Febbs Lett. Sep 29; 415(2): 163-168 (1997).

Pallavicini A., Zimbello R., Tiso N., Muraro T., Rampoldi L., Bortoluzzi S., Valle G., Lanfranchi G. and Danieli G.A.: 'The preliminary transcript map of a human skeletal muscle'. Human Molecular Genetics, Sep 6 (9): 1445-1450 (1997).

Tiso N., Rampoldi L., Pallavicini A., Zimbello R., Pandolfo D., Valle G., Lanfranchi G. and Danieli G.A.: 'Fine Mapping of Five Human Skeletal Muscle Genes: Alpha-Tropomyosin, Beta-Tropomyosin, Troponin-I Slow-Twitch, Troponin-I Fast-Twitch and Troponin-C Fast'. Biochemical and Biophysical Research Communications, 230, 347-350 (1997).

Muraro T., Stephan D., Tiso N., Zimbello R., Danieli G.A., Hoffman E.H., Valle G. and Lanfranchi G.: 'Chromosomal assignment of 115 expressed sequence tags (ESTs) from human skeletal muscle'. Cytogenet. Cell Genet. 76 (3-4), 144-152 (1997).

Tiso N., Pallavicini A., Muraro T., Zimbello R., Apolloni E., Valle G., Lanfranchi G. and Danieli G.A.: 'Chromosomal Localization of the Human Genes CPP32, Mch2, Mch3 and Ich-1, involved in Cellular Apoptosis'. Biochemical and Biophysical Research Communications 225, 983-989 (1996).

Albertin G., Rossi G.P., Majone F., Tiso N., Mattara A., G.A. Danieli, Pessina A. and Palu' G.: 'Fine Mapping of the Human Endothelin-Converting Enzyme by Fluorescent in Situ Hybridization and Radiation Hybrids'. Biochemical and Biophysical Research Communications 221: 682-687 (1996).

Rampazzo A., Nava a., Eberhard P., Vian E., Slomp P., Tiso N., Thiene G. and Danieli G.A.: 'A new locus for arrhythmogenic right ventricular cardiomyopathy (ARVD2 ) maps to chromosome 1q42-q43'. Human Molecular Genetics vol. 4: 2151-2154 (1995).