ZFIN ID: ZDB-PUB-160601-13
Development and Application of Functionalized Protein Binders in Multicellular Organisms
Bieli, D., Alborelli, I., Harmansa, S., Matsuda, S., Caussinus, E., Affolter, M.
Date: 2016
Source: International review of cell and molecular biology   325: 181-213 (Chapter)
Registered Authors: Affolter, Markus
Keywords: DARPins, Drosophila, mouse, nanobodies, protein manipulation, zebrafish
MeSH Terms:
  • Animals
  • Models, Molecular
  • Protein Binding
  • Proteins/chemistry
  • Proteins/metabolism*
  • Tissue Scaffolds
PubMed: 27241221 Full text @ Int. Rev. Cell Mol. Biol.
Protein-protein interactions are crucial for almost all biological processes. Studying such interactions in their native environment is critical but not easy to perform. Recently developed genetically encoded protein binders were shown to function inside living cells. These molecules offer a new, direct way to assess protein function, distribution and dynamics in vivo. A widely used protein binder scaffold are the so-called nanobodies, which are derived from the variable domain of camelid heavy-chain antibodies. Another commonly used scaffold, the DARPins, is based on Ankyrin repeats. In this review, we highlight how these binders can be functionalized in order to study proteins in vivo during the development of multicellular organisms. It is to be anticipated that many more applications for such synthetic protein binders will be developed in the near future.