ZFIN ID: ZDB-PUB-160521-6
Afferent Connectivity of the Zebrafish Habenulae
Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M.
Date: 2016
Source: Frontiers in neural circuits   10: 30 (Journal)
Registered Authors: Folgueira Otero, Monica, Hawkins, Tom, Wilson, Steve
Keywords: afferents, basal ganglia, connections, entopeduncular nucleus, habenula, posterior tuberculum, zebrafish
MeSH Terms:
  • Afferent Pathways/physiology*
  • Amino Acids/metabolism
  • Animals
  • Animals, Genetically Modified
  • Basal Ganglia
  • Brain Mapping*
  • Calbindin 2/genetics
  • Calbindin 2/metabolism
  • Embryo, Nonmammalian
  • Functional Laterality
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology*
  • Glutamic Acid/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Habenula/cytology
  • Habenula/growth & development
  • Habenula/physiology*
  • Larva
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Neurons/metabolism
  • Olfactory Bulb
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 27199671 Full text @ Front. Neural Circuits
The habenulae are bilateral nuclei located in the dorsal diencephalon that are conserved across vertebrates. Here we describe the main afferents to the habenulae in larval and adult zebrafish. We observe afferents from the subpallium, nucleus rostrolateralis, posterior tuberculum, posterior hypothalamic lobe, median raphe; we also see asymmetric afferents from olfactory bulb to the right habenula, and from the parapineal to the left habenula. In addition, we find afferents from a ventrolateral telencephalic nucleus that neurochemical and hodological data identify as the ventral entopeduncular nucleus (vENT), confirming and extending observations of Amo et al. (2014). Fate map and marker studies suggest that vENT originates from the diencephalic prethalamic eminence and extends into the lateral telencephalon from 48 to 120 hour post-fertilization (hpf). No afferents to the habenula were observed from the dorsal entopeduncular nucleus (dENT). Consequently, we confirm that the vENT (and not the dENT) should be considered as the entopeduncular nucleus "proper" in zebrafish. Furthermore, comparison with data in other vertebrates suggests that the vENT is a conserved basal ganglia nucleus, being homologous to the entopeduncular nucleus of mammals (internal segment of the globus pallidus of primates) by both embryonic origin and projections, as previously suggested by Amo et al. (2014).