PUBLICATION

A toolbox to study epidermal cell types in zebrafish

Authors
Eisenhoffer, G.T., Slattum, G., Ruiz, O.E., Otsuna, H., Bryan, C.D., Lopez, J., Wagner, D.S., Bonkowsky, J.L., Chien, C.B., Dorsky, R.I., Rosenblatt, J.
ID
ZDB-PUB-160507-10
Date
2017
Source
Journal of Cell Science   130(1): 269-277 (Journal)
Registered Authors
Bonkowsky, Joshua, Chien, Chi-Bin, Dorsky, Richard, Eisenhoffer, George, Otsuna, Hideo, Wagner, Daniel
Keywords
Epithelia, zebrafish, In vivo
MeSH Terms
  • Animals
  • Cell Death/drug effects
  • Cell Division/drug effects
  • Crosses, Genetic
  • Cytological Techniques/methods*
  • DNA-Binding Proteins/metabolism
  • Enhancer Elements, Genetic/genetics
  • Epidermis/cytology*
  • Epidermis/drug effects
  • Epidermis/ultrastructure
  • Epithelial Cells/cytology
  • Epithelial Cells/drug effects
  • Female
  • Gene Expression Regulation, Developmental/drug effects
  • Imaging, Three-Dimensional
  • Male
  • Morpholinos/pharmacology
  • Time Factors
  • Transcription Factors/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism
PubMed
27149923 Full text @ J. Cell Sci.
Abstract
Epithelia provide a critical protective barrier for our organs and are also the sites where most carcinomas form. Most studies on epithelia and carcinomas use cell culture or organisms where high-resolution live imaging is inaccessible without invasive techniques. Here, we introduce the developing zebrafish epidermis as an excellent in vivo model system for studying a living epithelium. We developed tools to fluorescently tag specific epithelial cell types and express genes in a mosaic fashion using five GAL4 lines identified from an enhancer trap screen. When crossed to a variety of UAS effector lines, we can now track, ablate or monitor single cells at sub-cellular resolution. Using photo-cleavable morpholino oligonucleotides that target GAL4, we can also express genes in a mosaic fashion at specific times during development. Together, this system provides an excellent in vivo alternative to tissue culture cells, without the intrinsic concerns of culture conditions or transformation, and enables the investigation of distinct cell types within living epithelial tissues.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes