ZFIN ID: ZDB-PERS-960805-108
Chien, Chi-Bin (Deceased)
URL: http://chien.neuro.utah.edu/
Affiliation: Chien Lab
Address: Department of Neurobiology and Anatomy University of Utah Medical Center 409 Wintrobe 20 North 1900 East Salt Lake City, UT 84132 USA
Country: United States
Phone: (801) 585-1701
Fax: (801) 581-4233


B.A., 1981, Johns Hopkins University.
Ph.D., 1991, California Institute of Technology, Jerry Pine's lab.
Postdoc, 1991-1995, University of California San Diego, Bill Harris's lab.
Postdoc, 1996-1997, Max Planck Institute for Developmental Biology, Tuebingen, Germany, Friedrich Bonhoeffer's lab.


My primary interest is in axon pathfinding: how does the growth cone smell its environment, then make and execute pathfinding decisions? The lab is working on the known retinotectal pathfinding mutants, characterizing novel growth cone guidance receptors and signal transduction molecules, and developing imaging techniques for observing individual growth cones in vivo.

We showed that the astray mutant has a defect in a zebrafish Roundabout homolog, and are continuing to study intensively the cell-biological function of Slit-Robo signaling. We have also cloned several of the topography mutants. In particular, we have found that boxer and dackel are defective in heparan sulfate proteoglycan synthesis, and are continuing to study how the lack of HSPGs leads to defects in optic tract sorting. Most recently, we found that nevermind is mutant in the cyfip2 gene, which encodes a protein in the WAVE complex that also binds to FMR1 family members, implicating one or both of these pathways in axon sorting in the optic tract and guidance on the tectum.

More recently we have broadened our interests to include vascular guidance (specifically, the role of Netrin1a signaling), dorsoventral patterning of the eye (Bmp and Wnt signaling), and eye morphogenesis (complex cell movements and their interplay with patterning).

I am also very interested in developing resources for the community, including DNA reagents, software, and fish. The Tol2kit is a set of plasmids for multisite Gateway cloning of Tol2 expression constructs, which provides a platform for facile exchange of plasmid constructs between labs. We are collaborating with computer scientists at Utah to develop new 3D visualization software (FluoRender), as well as software for tracking cell movements in 4D datasets (LongTracker).
We are currently conducting a Gal4 enhancer trap screen, for which we are documenting expression patterns by widefield and confocal microscopy; lines will be distributed through ZIRC.

Casey, M.A., Hill, J.T., Hoshijima, K., Bryan, C.D., Gribble, S.L., Brown, J.T., Chien, C.B., Yost, H.J., Kwan, K.M. (2021) Shutdown corner, a large deletion mutant isolated from a haploid mutagenesis screen in zebrafish. G3 (Bethesda). 12(3):
Wierson, W.A., Welker, J.M., Almeida, M.P., Mann, C.M., Webster, D.A., Torrie, M.E., Weiss, T.J., Kambakam, S., Vollbrecht, M.K., Lan, M., McKeighan, K.C., Levey, J., Ming, Z., Wehmeier, A., Mikelson, C.S., Haltom, J.A., Kwan, K.M., Chien, C.B., Balciunas, D., Ekker, S.C., Clark, K.J., Webber, B.R., Moriarity, B.S., Solin, S.L., Carlson, D.F., Dobbs, D.L., McGrail, M., Essner, J. (2020) Efficient targeted integration directed by short homology in zebrafish and mammalian cells. eLIFE. 9:
Eisenhoffer, G.T., Slattum, G., Ruiz, O.E., Otsuna, H., Bryan, C.D., Lopez, J., Wagner, D.S., Bonkowsky, J.L., Chien, C.B., Dorsky, R.I., Rosenblatt, J. (2017) A toolbox to study epidermal cell types in zebrafish. Journal of Cell Science. 130(1):269-277
Bryan, C.D., Chien, C.B., Kwan, K.M. (2016) Loss of laminin alpha 1 results in multiple structural defects and divergent effects on adhesion during vertebrate optic cup morphogenesis. Developmental Biology. 416(2):324-37
Gaynes, J.A., Otsuna, H., Campbell, D.S., Manfredi, J.P., Levine, E.M., Chien, C.B. (2015) The RNA Binding Protein Igf2bp1 Is Required for Zebrafish RGC Axon Outgrowth In Vivo. PLoS One. 10:e0134751
Otsuna, H., Hutcheson, D., Duncan, R., McPherson, A., Scoresby, A., Gaynes, B., Tong, Z., Fujimoto, E., Kwan, K., Chien, C., Dorsky, R. (2015) High-resolution analysis of CNS expression patterns in zebrafish Gal4 enhancer-trap lines. Developmental Dynamics : an official publication of the American Association of Anatomists. 244(6):785-96
Hutcheson, D., Otsuna, H., Chien, C.B., Dorsky, R. (2014) Enhancer Trap Mutants Gal4 Project. ZFIN Direct Data Submission.
Jain, R.A., Bell, H., Lim, A., Chien, C.B., Granato, M. (2014) Mirror movement-like defects in startle behavior of zebrafish dcc mutants are caused by aberrant midline guidance of identified descending hindbrain neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience. 34:2898-909
Gassman, A., Hao le, T., Bhoite, L., Bradford, C.L., Chien, C.B., Beattie, C.E, and Manfredi, J.P. (2013) Small molecule suppressors of Drosophila Kinesin deficiency rescue motor axon development in a zebrafish model of spinal muscular atrophy. PLoS One. 8(9):e74325
Nguyen, T.K., Tran, V.M., Venkataswamy, S., Eriksson, I., Kojima, A., Koketsu, M., Loganathan, D., Kjellén, L., Dorsky, R.I., Chien, C.B., and Kuberan, B. (2013) Dimerized glycosaminoglycan chains increase FGF signaling during zebrafish development. ACS Chemical Biology. 8(5):939-48
Poulain, F.E., and Chien, C.B. (2013) Proteoglycan-mediated axon degeneration corrects pretarget topographic sorting errors. Neuron. 78(1):49-56
Kruse-Bend, R., Rosenthal, J., Quist, T.S., Veien, E.S., Fuhrmann, S., Dorsky, R.I., and Chien, C.B. (2012) Extraocular ectoderm triggers dorsal retinal fate during optic vesicle evagination in zebrafish. Developmental Biology. 371(1):57-65
Wang, X., Kopinke, D., Lin, J., McPherson, A.D., Duncan, R.N., Otsuna, H., Moro, E., Hoshijima, K., Grunwald, D.J., Argenton, F., Chien, C.B., Murtaugh, L.C., and Dorsky, R.I. (2012) Wnt signaling regulates postembryonic hypothalamic progenitor differentiation. Developmental Cell. 23(3):624-636
Hörndli C.S., and Chien, C.B. (2012) Sonic hedgehog is indirectly required for intraretinal axon pathfinding by regulating chemokine expression in the optic stalk. Development (Cambridge, England). 139(14):2604-2613
Bonner, J., Letko, M., Nikolaus, O.B., Krug, L., Cooper, A., Chadwick, B., Conklin, P., Lim, A., Chien, C.B., and Dorsky, R.I. (2012) Midline crossing is not required for subsequent pathfinding decisions in commissural neurons. Neural Development. 7(1):18
Eisenhoffer, G.T., Loftus, P.D., Yoshigi, M., Otsuna, H., Chien, C.B., Morcos, P.A., and Rosenblatt, J. (2012) Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia. Nature. 484(7395):546-549
Kwan, K.M., Otsuna, H., Kidokoro, H., Carney, K.R., Saijoh, Y., and Chien, C.B. (2012) A complex choreography of cell movements shapes the vertebrate eye. Development (Cambridge, England). 139(2):359-372
Xing, L., Hoshijima, K., Grunwald, D.J., Fujimoto, E., Quist, T.S., Sneddon, J., Chien, C.B., Stevenson, T.J., and Bonkowsky, J.L. (2012) Zebrafish foxP2 Zinc Finger Nuclease Mutant Has Normal Axon Pathfinding. PLoS One. 7(8):e43968
Fujimoto, E., Gaynes, B., Brimley, C.J., Chien, C.B., and Bonkowsky, J.L. (2011) Gal80 intersectional regulation of cell-type specific expression in vertebrates. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(10):2324-34
Lim, A.H., Suli, A., Yaniv, K., Weinstein, B., Li, D.Y., and Chien, C.B. (2011) Motoneurons are essential for vascular pathfinding. Development (Cambridge, England). 138(17):3847-3857
Fujimoto, E., Stevenson, T.J., Chien, C.B., and Bonkowsky, J.L. (2011) Identification of a Dopaminergic Enhancer Indicates Complexity in Vertebrate Dopamine Neuron Phenotype Specification. Developmental Biology. 352(2):393-404
Choi, J.H., Law, M.Y., Chien, C.B., Link, B.A., and Wong, R.O. (2010) In vivo development of dendritic orientation in wild-type and mislocalized retinal ganglion cells. Neural Development. 5:29
Wyatt, C., Ebert, A., Reimer, M.M., Rasband, K., Hardy, M., Chien, C.B., Becker, T., and Becker, C.G. (2010) Analysis of the astray/robo2 Zebrafish Mutant Reveals that Degenerating Tracts Do Not Provide Strong Guidance Cues for Regenerating Optic Axons. The Journal of neuroscience : the official journal of the Society for Neuroscience. 30(41):13838-13849
Pittman, A.J., Gaynes, J.A., and Chien, C.B. (2010) nev (cyfip2) Is required for retinal lamination and axon guidance in the zebrafish retinotectal system. Developmental Biology. 344(2):784-794
Fredj, N.B., Hammond, S., Otsuna, H., Chien, C.B., Burrone, J., and Meyer, M.P. (2010) Synaptic activity and activity-dependent competition regulates axon arbor maturation, growth arrest, and territory in the retinotectal projection. The Journal of neuroscience : the official journal of the Society for Neuroscience. 30(32):10939-10951
Poulain, F.E., Gaynes, J.A., Hörndli, C.S., Law, M.Y., and Chien, C.B. (2010) Chapter 1 - Analyzing retinal axon guidance in zebrafish. Methods in cell biology. 100:2-26
Wan, Y., Otsuna, H., Chien, C.B., and Hansen, C. (2009) An interactive visualization tool for multi-channel confocal microscopy data in neurobiology research. IEEE transactions on visualization and computer graphics. 15(6):1489-1496
Kastenhuber, E., Kern U., Bonkowsky, J.L., Chien, C.B., Driever, W., and Schweitzer, J. (2009) Netrin-DCC, Robo-Slit, and heparan sulfate proteoglycans coordinate lateral positioning of longitudinal dopaminergic diencephalospinal axons. The Journal of neuroscience : the official journal of the Society for Neuroscience. 29(28):8914-8926
Willardsen, M.I., Suli, A., Pan, Y., Marsh-Armstrong, N., Chien, C.B., El-Hodiri, H., Brown, N.L., Moore, K.B., and Vetter, M.L. (2009) Temporal regulation of Ath5 gene expression during eye development. Developmental Biology. 326(2):471-481
Veien, E.S., Rosenthal, J.S., Kruse-Bend, R.C., Chien, C.B., and Dorsky, R.I. (2008) Canonical Wnt signaling is required for the maintenance of dorsal retinal identity. Development (Cambridge, England). 135(24):4101-4111
Bonkowsky, J.L., Wang, X., Fujimoto, E., Lee, J.E., Chien, C.B., and Dorsky, R.I. (2008) Domain-specific regulation of foxP2 CNS expression by lef1. BMC Developmental Biology. 8:103
Pittman, A.J., Law, M.Y., and Chien, C.B. (2008) Pathfinding in a large vertebrate axon tract: isotypic interactions guide retinotectal axons at multiple choice points. Development (Cambridge, England). 135(17):2865-2871
Clément, A., Wiweger, M., von der Hardt, S., Rusch, M.A., Selleck, S.B., Chien, C.B., and Roehl, H.H. (2008) Regulation of zebrafish skeletogenesis by ext2/dackel and papst1/pinscher. PLoS Genetics. 4(7):e1000136
Navankasattusas, S., Whitehead, K.J., Suli, A., Sorensen, L.K., Lim, A.H., Zhao, J., Park, K.W., Wythe, J.D., Thomas, K.R., Chien, C.B., and Li, D.Y. (2008) The netrin receptor UNC5B promotes angiogenesis in specific vascular beds. Development (Cambridge, England). 135(4):659-667
Kwan, K.M., Fujimoto, E., Grabher, C., Mangum, B.D., Hardy, M.E., Campbell, D.S., Parant, J.M., Yost, H.J., Kanki, J.P., and Chien, C.B. (2007) The Tol2kit: A multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Developmental Dynamics : an official publication of the American Association of Anatomists. 236(11):3088-3099
Hardy, M.E., Ross, L.V., and Chien, C.B. (2007) Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron. Developmental Dynamics : an official publication of the American Association of Anatomists. 236(11):3071-3076
Campbell, D.S., Stringham, S.A., Timm, A., Xiao, T., Law, M.Y., Baier, H., Nonet, M.L., and Chien, C.B. (2007) Slit1a inhibits retinal ganglion cell arborization and synaptogenesis via Robo2-dependent and -independent pathways. Neuron. 55(2):231-245
Ivanov, I.P., Pittman, A.J., Chien, C.B., Gesteland, R.F., and Atkins, J.F. (2007) Novel antizyme gene in Danio rerio expressed in brain and retina. Gene. 387(1-2):87-92
Chalasani, S.H., Sabol, A., Xu, H., Gyda, M.A., Rasband, K., Granato, M., Chien, C.B., and Raper, J.A. (2007) Stromal cell-derived factor-1 antagonizes slit/robo signaling in vivo. The Journal of neuroscience : the official journal of the Society for Neuroscience. 27(5):973-980
Ekker, S.C., Stemple, D.L., Clark, M., Chien, C.B., Rasooly, R.S., and Javois, L.C. (2007) Zebrafish genome project: bringing new biology to the vertebrate genome field. Zebrafish. 4(4):239-251
Suli, A., Mortimer, N., Shepherd, I., and Chien, C.B. (2006) Netrin/DCC signaling controls contralateral dendrites of octavolateralis efferent neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience. 26(51):13328-13337
Mumm, J.S., Williams, P.R., Godinho, L., Koerber, A., Pittman, A.J., Roeser, T., Chien, C.B., Baier, H., and Wong, R.O. (2006) In vivo imaging reveals dendritic targeting of laminated afferents by zebrafish retinal ganglion cells. Neuron. 52(4):609-621
Zolessi, F.R., Poggi, L., Wilkinson, C.J., Chien, C.B., and Harris, W.A. (2006) Polarization and orientation of retinal ganglion cells in vivo. Neural Development. 1:2
Wilson, B.D., Ii, M., Park, K.W., Suli, A., Sorensen, L.K., Larrieu-Lahargue, F., Urness, L.D., Suh, W., Asai, J., Kock, G.A., Thorne, T., Silver, M., Thomas, K.R., Chien, C.B., Losordo, D.W., and Li, D.Y. (2006) Netrins Promote Developmental and Therapeutic Angiogenesis. Science (New York, N.Y.). 313(5787):640-644
Fricke, C., and Chien, C.B. (2005) Cloning of full-length zebrafish dcc and expression analysis during embryonic and early larval development. Developmental Dynamics : an official publication of the American Association of Anatomists. 234(3):732-739
Park, K.W., Urness, L.D., Senchuk, M.M., Colvin, C.J., Wythe, J.D., Chien, C.B., and Li, D.Y. (2005) Identification of new netrin family members in zebrafish: Developmental expression of netrin2 and netrin4. Developmental Dynamics : an official publication of the American Association of Anatomists. 234(3):726-731
Bonkowsky, J.L., and Chien, C.B. (2005) Molecular cloning and developmental expression of foxP2 in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 234(3):740-746
Barresi, M.J., Hutson, L.D., Chien, C.B., and Karlstrom, R.O. (2005) Hedgehog regulated Slit expression determines commissure and glial cell position in the zebrafish forebrain. Development (Cambridge, England). 132(16):3643-3656
Miyasaka, N., Sato, Y., Yeo, S.Y., Hutson, L.D., Chien, C.B., Okamoto, H., and Yoshihara, Y. (2005) Robo2 is required for establishment of a precise glomerular map in the zebrafish olfactory system. Development (Cambridge, England). 132(6):1283-1293
Lee, J.S., von der Hardt, S., Rusch, M.A., Stringer, S.E., Stickney, H.L., Talbot, W.S., Geisler, R., Nüsslein-Volhard, C., Selleck, S.B., Chien, C.B., and Roehl, H. (2004) Axon Sorting in the Optic Tract Requires HSPG Synthesis by ext2 (dackel) and extl3 (boxer). Neuron. 44(6):947-960
Lee, J.S., and Chien, C.B. (2004) When sugars guide axons: insights from heparan sulphate proteoglycan mutants. Nature reviews. Genetics. 5(12):923-935
Yeo, S.Y., Miyashita, T., Fricke, C., Little, M.H., Yamada, T., Kuwada, J.Y., Huh, T.L., Chien, C.B., and Okamoto, H. (2004) Involvement of Islet-2 in the Slit signaling for axonal branching and defasciculation of the sensory neurons in embryonic zebrafish. Mechanisms of Development. 121(4):315-324
Hutson, L.D., Jurynec, M.J., Yeo, S.Y., Okamoto, H., and Chien, C.B. (2003) Two divergent slit1 genes in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 228(3):358-369
Rasband, K., Hardy, M., and Chien, C.-B. (2003) Generating X: formation of the optic chiasm. Neuron. 39(6):885-888
Chien, C.-B. and Piotrowski, T. (2002) How the lateral line gets its glia. Trends in neurosciences. 25(11):544-546
Kawahara, A., Chien, C.-B., and Dawid, I.B. (2002) The homeobox gene mbx is involved in eye and tectum development. Developmental Biology. 248(1):107-117
Hutson, L.D. and Chien, C.-B. (2002) Wiring the zebrafish: axon guidance and synaptogenesis. Current opinion in neurobiology. 12(1):87-92
Hutson, L.D. and Chien, C.-B. (2002) Pathfinding and Error Correction by Retinal Axons: The Role of astray/robo2. Neuron. 33(2):205-217
Lee, J.S., Ray, R., and Chien, C.B. (2001) Cloning and expression of three zebrafish roundabout homologs suggest roles in axon guidance and cell migration. Developmental Dynamics : an official publication of the American Association of Anatomists. 221(2):216-230
Fricke, C., Lee, J.S., Geiger-Rudolph, S., Bonhoeffer, F., and Chien, C.-B. (2001) astray, a zebrafish roundabout homolog required for retinal axon guidance. Science (New York, N.Y.). 292(5516):507-510
Hutson, L.D. and Chien, C.-B. (2001) Error correction in axon guidance: astray/Robo-2 is required during development of the zebrafish retinotectal pathway. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 15(5):A1071

Chondroitin sulfate disrupts axon pathfinding in the optic tract and alters growth cone dynamics. Walz A, Anderson RB, Irie A, Chien CB, Holt CE. J Neurobiol. 53(3):330-42 (2002)

'Why does the growth cone cross the road? (minireview)' CB Chien. Neuron 20:3-6 (1998).

'PY in the fly: receptor-like tyrosine phosphatases in axonal pathfinding (minireview).' CB Chien. Neuron 16:1065-1068 (1996).

'Signal transduction in vertebrate growth cones navigating in vivo.' CB Chien and WA Harris. Perspect Dev Neurobiol, 4: 253-266 (1996).

'Embryonic taste buds develop in the absence of innervation.' LA Barlow, CB Chien, and RG Northcutt. Development 122: 1103-1111 (1996).

'Absence of topography in precociously innervated tecta.' CB Chien, EM Cornel, and CE Holt. Development 121: 2621-2631 (1995).

'Axonal guidance from retina to tectum in embryonic Xenopus.' CB Chien and WA Harris. Curr Top Dev Biol, 29: 135-169 (1994).

'Navigational errors made by growth cones without filopodia in the embryonic Xenopus brain.' CB Chien, DE Rosenthal, WA Harris, and CE Holt. Neuron 11: 237-251 (1993).