ZFIN ID: ZDB-PUB-160426-6
A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.
Hsu, C.H., Lin, J.S., Lai, K.P., Li, J.W., Chan, T.F., You, M.S., Tse, W.K., Jiang, Y.J.
Date: 2015
Source: Scientific Reports   5: 10673 (Journal)
Registered Authors: Hsu, Chia-Hao, Jiang, Yun-Jin, Lin, Ji-Sheng, Tse, Ka Fai William, You, May-su
Keywords: Development, Mesoderm
MeSH Terms:
  • Alleles*
  • Apoptosis/genetics
  • Biomarkers
  • Chromosome Deletion*
  • Forkhead Transcription Factors/chemistry
  • Forkhead Transcription Factors/genetics*
  • Forkhead Transcription Factors/metabolism
  • Genetic Loci
  • Homozygote
  • Mutation
  • Organogenesis/genetics
  • Phenotype
  • Protein Interaction Domains and Motifs
  • Somites/metabolism*
  • Somites/pathology
  • Ubiquitin-Protein Ligases/chemistry
  • Ubiquitin-Protein Ligases/genetics*
  • Ubiquitin-Protein Ligases/metabolism
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 26039894 Full text @ Sci. Rep.

mibnn2002, found from an allele screen, showed early segmentation defect and severe cell death phenotypes, which are different from previously known mib mutants. Despite distinct morphological phenotypes, the typical mib molecular phenotypes: her4 down-regulation, neurogenic phenotype and cold sensitive dlc expression pattern, still remained. The linkage analysis also indicated that mibnn2002 is a new mib allele. Failure of specification in anterior 7-10 somites is likely due to lack of foxc1a expression in mibnn2002 homozygotes. Somites and somite markers gradually appeared after 7-10 somite stage, suggesting that foxc1a is only essential for the formation of anterior 7-10 somites. Apoptosis began around 16-somite stage with p53 up-regulation. To find the possible links of mib, foxc1a and apoptosis, transcriptome analysis was employed. About 140 genes, including wnt3a, foxc1a and mib, were not detected in the homozygotes. Overexpression of foxc1a mRNA in mibnn2002 homozygotes partially rescued the anterior somite specification. In the process of characterizing mibnn2002 mutation, we integrated the scaffolds containing mib locus into chromosome 2 (or linkage group 2, LG2) based on synteny comparison and transcriptome results. Genomic PCR analysis further supported the conclusion and showed that mibnn2002 has a chromosomal deletion with the size of about 9.6 Mbp.