PUBLICATION
Changes in neurotransmitter profiles during early zebrafish (Danio rerio) development and after pesticide exposure
- Authors
- Tufi, S., Leonards, P.E., Lamoree, M.H., De Boer, J., Legler, J., Legradi, J.
- ID
- ZDB-PUB-160213-12
- Date
- 2016
- Source
- Environmental science & technology 50(6): 3222-30 (Journal)
- Registered Authors
- Legler, Juliette, Legradi, Jessica
- Keywords
- none
- MeSH Terms
-
- Animals
- Central Nervous System
- Chromatography, Liquid/methods
- Ecotoxicology/methods*
- Embryo, Nonmammalian
- Female
- Larva/drug effects
- Larva/metabolism
- Neurotransmitter Agents/metabolism*
- Pesticides/toxicity*
- Tandem Mass Spectrometry
- Water Pollutants, Chemical/toxicity*
- Zebrafish*/embryology
- Zebrafish*/metabolism
- PubMed
- 26866575 Full text @ Env. Sci. Tech.
Citation
Tufi, S., Leonards, P.E., Lamoree, M.H., De Boer, J., Legler, J., Legradi, J. (2016) Changes in neurotransmitter profiles during early zebrafish (Danio rerio) development and after pesticide exposure. Environmental science & technology. 50(6):3222-30.
Abstract
During early development, neurotransmitters are important stimulants for the development of the central nervous system. Although the development of different neuronal cell types during early zebrafish (Danio rerio) development is well studied, little is known of the levels of neurotransmitters, their precursors and metabolites during development and how these levels are affected by exposure to environmental contaminants. A method based on hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry has been applied for the first time to zebrafish embryos and larvae to study five neurotransmitter systems in parallel, including the dopaminergic/andrenergic, glutaminergic/GABAnergic, serotoninergic, histaminergic and cholinergic systems. Our method enables the quantification of neurotransmitters, their precursors and metabolites in whole zebrafish from the period of zygote to free swimming larvae 6 days post fertilization (dpf). We observed a developmental stage-dependent pattern, with clear differences between the first two days of development and the following days. Whereas the neurotransmitter levels steadily increased, the precursors showed a peak at 3 dpf. After exposure to several pesticides, significant differences in concentrations of neurotransmitters and precursors were observed. Our study revealed new insights about neurotransmitter systems during early zebrafish development and showed the usefulness of our approach for environmental neurotoxicity studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping