ZFIN ID: ZDB-PERS-960805-325
Legler, Juliette
Email: juliette.legler@ivm.falw.vu.nl
URL:
Affiliation: Legler Lab
Address: Institute for Environmental Studies (IVM) Vrije University De Boelelaan 1087 Amsterdam, 1081 HV Netherlands
Country: Netherlands
Phone: 31-20-598-9535
Fax: 31-20-598-9553
ORCID ID:


BIOGRAPHY AND RESEARCH INTERESTS
Juliette Legler is a toxicologist with training in environmental sciences, aquatic ecotoxicology and molecular biology. She has been employed since 2001 by the IVM and was recently awarded an NWO-Veni grant to initiate research in toxicogenomics in (transgenic) zebrafish. In addition, her current research involves the application of in vitro bioassays to determine the effects of organohalogenated compounds on endocrine status (EU project COMPARE), as well as mixture toxicity of low concentrations of estrogenic compounds (EU project ACE). During her PhD research, two novel bioassays were developed as biomarkers of exposure to natural and xenobiotic estrogens in the environment. Firstly, the estrogen receptor (ER)-mediated chemical activated luciferase reporter gene expression (ER-CALUX) assay was developed by stable transfection of an ER-regulated luciferase reporter gene in the T47D breast cancer cell line. Secondly, a functional in vivo reporter gene assay using transgenic zebrafish was developed for the rapid determination of the effects of estrogenic chemicals on sensitive life stages and target tissues.
During post-doctoral research, the ability of the ER-CALUX assay to predict in vivo effects in the transgenic zebrafish was researched, as well as differences in ligand affinity between human and zebrafish ERs. Her research also included the determination of the biological relevance of the transgenic zebrafish model, by examining the relationship between reporter gene response by endocrine disrupting chemicals and effects on reproduction and gonad differentiation in zebrafish.


PUBLICATIONS
Garoche, C., Boulahtouf, A., Grimaldi, M., Chiavarina, B., Toporova, L., den Broeder, M.J., Legler, J., Bourguet, W., Balaguer, P. (2021) Interspecies Differences in Activation of Peroxisome Proliferator-Activated Receptor γ by Pharmaceutical and Environmental Chemicals. Environmental science & technology. 55(24):16489-16501
den Broeder, M.J., Ballangby, J., Kamminga, L.M., Aleström, P., Legler, J., Lindeman, L.C., Kamstra, J.H. (2020) Inhibition of methyltransferase activity of enhancer of zeste 2 leads to enhanced lipid accumulation and altered chromatin status in zebrafish. Epigenetics & chromatin. 13:5
Kamstra, J.H., Hurem, S., Martin, L.M., Lindeman, L.C., Legler, J., Oughton, D., Salbu, B., Brede, D.A., Lyche, J.L., Aleström, P. (2018) Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish. Scientific Reports. 8:15373
Kopp, R., Martínez, I.O., Legradi, J., Legler, J. (2017) Exposure to endocrine disrupting chemicals perturbs lipid metabolism and circadian rhythms. Journal of environmental sciences (China). 62:133-137
Legradi, J., Pomeren, M.V., Dahlberg, A.K., Legler, J. (2017) Effects of Hydroxylated Polybrominated Diphenyl Ethers in Developing Zebrafish Are Indicative of Disruption of Oxidative Phosphorylation. International Journal of Molecular Sciences. 18(5)
den Broeder, M.J., Moester, M.J.B., Kamstra, J.H., Cenijn, P.H., Davidoiu, V., Kamminga, L.M., Ariese, F., de Boer, J.F., Legler, J. (2017) Altered Adipogenesis in Zebrafish Larvae Following High Fat Diet and Chemical Exposure Is Visualised by Stimulated Raman Scattering Microscopy. International Journal of Molecular Sciences. 18(4)
Kamstra, J.H., Sales, L.B., Aleström, P., Legler, J. (2017) Differential DNA methylation at conserved non-genic elements and evidence for transgenerational inheritance following developmental exposure to mono(2-ethylhexyl) phthalate and 5-azacytidine in zebrafish. Epigenetics & chromatin. 10:20
Aragon, A., Legradi, J., Ballesteros-Gómez, A., Legler, J., van Velzen, M., de Boer, J., Leonards, P. (2017) Determination of monoamine neurotransmitters in zebrafish (Danio rerio) by gas chromatography coupled to mass spectrometry with a two-step derivatization. Analytical and bioanalytical chemistry. 409(11):2931-2939
Kopp, R., Billecke, N., Legradi, J., den Broeder, M., Parekh, S.H., Legler, J. (2016) Bringing obesity to light: Rev-erbα, a central player in light-induced adipogenesis in the zebrafish?. International journal of obesity (2005). 40(5):824-32
Kopp, R., Legler, J., Legradi, J. (2016) Alterations in locomotor activity of feeding zebrafish larvae as a consequence of exposure to different environmental factors. Environmental science and pollution research international. 25(5):4085-4093
Tufi, S., Leonards, P.E., Lamoree, M.H., De Boer, J., Legler, J., Legradi, J. (2016) Changes in neurotransmitter profiles during early zebrafish (Danio rerio) development and after pesticide exposure. Environmental science & technology. 50(6):3222-30
Dahlberg, A.K., Bignert, A., Legradi, J., Legler, J., Asplund, L. (2016) Anthropogenic and naturally produced brominated substances in Baltic herring (Clupea harengus membras) from two sites in the Baltic Sea. Chemosphere. 144:2408-2414
Bouwmeester, M.C., Ruiter, S., Lommelaars, T., Sippel, J., Hodemaekers, H.M., van den Brandhof, E.J., Pennings, J.L., Kamstra, J.H., Jelinek, J., Issa, J.J., Legler, J., van der Ven, L.T. (2016) Zebrafish embryos as a screen for DNA methylation modifications after compound exposure. Toxicology and applied pharmacology. 291:84-96
Kamstra, J.H., Aleström, P., Kooter, J.M., Legler, J. (2015) Zebrafish as a model to study the role of DNA methylation in environmental toxicology. Environmental science and pollution research international. 22(21):16262-76
Legradi, J., El Abdellaoui, N., van Pomeren, M., Legler, J. (2015) Comparability of behavioural assays using zebrafish larvae to assess neurotoxicity. Environmental science and pollution research international. 22(21):16277-89
Kamstra, J.H., Løken, M., Aleström, P., Legler, J. (2015) Dynamics of DNA Hydroxymethylation in Zebrafish. Zebrafish. 12(3):230-7
Den Broeder, M.J., Kopylova, V.A., Kamminga, L.M., Legler, J. (2015) Zebrafish as a Model to Study the Role of Peroxisome Proliferating-Activated Receptors in Adipogenesis and Obesity. PPAR research. 2015:358029
Busquet, F., Strecker, R., Rawlings, J.M., Belanger, S.E., Braunbeck, T., Carr, G.J., Cenijn, P., Fochtman, P., Gourmelon, A., Hübler, N., Kleensang, A., Knöbel, M., Kussatz, C., Legler, J., Lillicrap, A., Martínez-Jerónimo, F., Polleichtner, C., Rzodeczko, H., Salinas, E., Schneider, K.E., Scholz, S., Brandhof, E.J., van der Ven, L.T., Walter-Rohde, S., Weigt, S., Witters, H., Halder, M. (2014) OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing. Regulatory toxicology and pharmacology : RTP. 69(3):496-511
Hawliczek, A., Nota, B., Cenijn, P., Kamstra, J., Pieterse, B., Winter, R., Winkens, K., Hollert, H., Segner, H., and Legler, J. (2012) Developmental toxicity and endocrine disrupting potency of 4-azapyrene, benzo[b]fluorene and retene in the zebrafish Danio rerio. Reproductive toxicology (Elmsford, N.Y.). 33(2):213-223
Legler, J., Velzen, M.V., Cenijn, P., Houtman, C., Lamoree, M., and Wegener, J. (2011) Effect-directed analysis of municipal landfill soil reveals novel developmental toxicants in the zebrafish Danio rerio. Environmental science & technology. 45(19):8552-8
Busch, W., Duis, K., Fenske, M., Maack, G., Legler, J., Padilla, S., Strähle, U., Witters, H., and Scholz, S. (2011) The zebrafish embryo model in toxicology and teratology. Reproductive toxicology (Elmsford, N.Y.). 31(4):585-588
van Boxtel, A.L., Gansner, J.M., Hakvoort, H.W., Snell, H., Legler, J., and Gitlin, J.D. (2011) Lysyl oxidase-like 3b is Critical for Cartilage Maturation During Zebrafish Craniofacial Development. Matrix biology : journal of the International Society for Matrix Biology. 30(3):178-87
van Boxtel, A.L., Pieterse, B., Cenijn, P., Kamstra, J.H., Brouwer, A., van Wieringen, W., de Boer, J., and Legler, J. (2010) Dithiocarbamates Induce Craniofacial Abnormalities and Downregulate sox9a during Zebrafish Development. Toxicological sciences : an official journal of the Society of Toxicology. 117(1):209-217
van Boxtel, A.L., Kamstra, J.H., Fluitsma, D.M., and Legler, J. (2010) Dithiocarbamates are teratogenic to developing zebrafish through inhibition of lysyl oxidase activity. Toxicology and applied pharmacology. 244(2):156-161
van Boxtel, A.L., Kamstra, J.H., Cenijn, P.H., Pieterse, B., Wagner, J.M., Antink, M., Krab, K., van der Burg, B., Marsh, G., Brouwer, A., and Legler, J. (2008) Microarray analysis reveals a mechanism of phenolic polybrominated diphenylether toxicity in zebrafish. Environmental science & technology. 42(5):1773-1779
Bogers, R., Mutsaerds, E., Druke, J., De Roode, D.F., Murk, A.J., Van Der Burg, B., and Legler, J. (2006) Estrogenic endpoints in fish early life-stage tests: luciferase and vitellogenin induction in estrogen-responsive transgenic zebrafish. Environmental toxicology and chemistry. 25(1):241-247
Schreurs, R.H., Legler, J., Artola-Garicano, E., Sinnige, T.L., Lanser, P.H., Seinen, W., and Van der Burg, B. (2004) In vitro and in vivo antiestrogenic effects of polycyclic musks in zebrafish. Environmental science & technology. 38(4):997-1002
Legler, J., Zeinstra, L.M., Schuitemaker, F., Lanser, P.H., Bogerd, J., Brouwer, A., Vethaak, A.D., De Voogt, P., Murk, A.J., and Van der Burg, B. (2002) Comparison of in vivo and in vitro reporter gene assays for short-term screening of estrogenic activity. Environmental science & technology. 36(20):4410-4415
Legler, J., Broekhof, J.L.M., Brouwer, A., Lanser, P.H., Murk, A.J., Van der Saag, P.T., Vethaak, A.D., Wester, P., Zivkovic, D., and Van der Burg, B. (2000) A novel in vivo bioassay for (xeno-)estrogens using transgenic zebrafish. Environmental science & technology. 34(20):4439-4444

NON-ZEBRAFISH PUBLICATIONS
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.