ZFIN ID: ZDB-PUB-160126-2
A genetically targetable near-infrared photosensitizer
He, J., Wang, Y., Missinato, M.A., Onuoha, E., Perkins, L.A., Watkins, S.C., St Croix, C.M., Tsang, M., Bruchez, M.P.
Date: 2016
Source: Nature Methods   13(3): 263-8 (Journal)
Registered Authors: Tsang, Michael, Wang, Yi
Keywords: Chemical tools, Optogenetics, Self-renewal
MeSH Terms:
  • Apoptosis/physiology
  • Apoptosis/radiation effects*
  • Dose-Response Relationship, Radiation
  • HEK293 Cells
  • Humans
  • Infrared Rays*
  • Photochemotherapy/methods*
  • Photosensitizing Agents/chemistry*
  • Photosensitizing Agents/radiation effects*
  • Radiation Dosage
  • Recombinant Proteins/genetics*
  • Recombinant Proteins/therapeutic use
PubMed: 26808669 Full text @ Nat. Methods
Upon illumination, photosensitizer molecules produce reactive oxygen species that can be used for functional manipulation of living cells, including protein inactivation, targeted-damage introduction and cellular ablation. Photosensitizers used to date have been either exogenous, resulting in delivery and removal challenges, or genetically encoded proteins that form or bind a native photosensitizing molecule, resulting in a constitutively active photosensitizer inside the cell. We describe a genetically encoded fluorogen-activating protein (FAP) that binds a heavy atom-substituted fluorogenic dye, forming an 'on-demand' activated photosensitizer that produces singlet oxygen and fluorescence when activated with near-infrared light. This targeted and activated photosensitizer (TAPs) approach enables protein inactivation, targeted cell killing and rapid targeted lineage ablation in living larval and adult zebrafish. The near-infrared excitation and emission of this FAP-TAPs provides a new spectral range for photosensitizer proteins that could be useful for imaging, manipulation and cellular ablation deep within living organisms.